Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Susan Heimbecher; Yung Chi Lee; S. Esmail Tabibi; Samuel H. Yalkowsky

doi:10.1016/S0378-4347(96)00452-5

Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Susan Heimbecher, Yung Chi Lee, S. Esmail Tabibi, Samuel H. Yalkowsky

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid high-performance liquid chromatographic method for determination of the dansyl derivative of pentaazapentacosane (PAPC) pentahydrochloride has been developed. The chromatographic system uses a reversed-phase C₈ column, a mobile phase of acetic acid buffer and acetonitrile and UV detection. The dansylation conditions were optimized with a pH of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PAPC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess dansyl chloride only perdansyl PAPC was found.

Original language	English (US)
Pages (from-to)	173-178
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Applications
Volume	691
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(96)00452-5
State	Published - Mar 28 1997

Keywords

Dansylation
Pentaazapentacosane pentahydrochloride
Polyamines

ASJC Scopus subject areas

General Chemistry

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10.1016/S0378-4347(96)00452-5

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title = "Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride",

abstract = "A rapid high-performance liquid chromatographic method for determination of the dansyl derivative of pentaazapentacosane (PAPC) pentahydrochloride has been developed. The chromatographic system uses a reversed-phase C8 column, a mobile phase of acetic acid buffer and acetonitrile and UV detection. The dansylation conditions were optimized with a pH of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PAPC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess dansyl chloride only perdansyl PAPC was found.",

keywords = "Dansylation, Pentaazapentacosane pentahydrochloride, Polyamines",

author = "Susan Heimbecher and Lee, {Yung Chi} and Tabibi, {S. Esmail} and Yalkowsky, {Samuel H.}",

note = "Funding Information: work was supported under Contract No. NOl-CM-27757 from the National Cancer Institute.",

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AU - Lee, Yung Chi

AU - Tabibi, S. Esmail

AU - Yalkowsky, Samuel H.

N1 - Funding Information: work was supported under Contract No. NOl-CM-27757 from the National Cancer Institute.

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AB - A rapid high-performance liquid chromatographic method for determination of the dansyl derivative of pentaazapentacosane (PAPC) pentahydrochloride has been developed. The chromatographic system uses a reversed-phase C8 column, a mobile phase of acetic acid buffer and acetonitrile and UV detection. The dansylation conditions were optimized with a pH of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PAPC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess dansyl chloride only perdansyl PAPC was found.

KW - Dansylation

KW - Pentaazapentacosane pentahydrochloride

KW - Polyamines

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Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Abstract

Keywords

ASJC Scopus subject areas

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