Depth random-access two-photon Bessel light-sheet imaging in brain tissue

Dongli Xu, Jun B. Ding, Leilei Peng

Research output: Contribution to journalArticlepeer-review


Two-photon light-sheet fluorescence microscopy enables high-resolution imaging of neural activity in brain tissue at a high frame rate. Traditionally, light-sheet microscopy builds up a 3D stack by multiple depth scans with uniform spatial intervals, which substantially limits the volumetric imaging speed. Here, we introduce the depth random-access light-sheet microscopy, allowing rapid switching scanning depth for light-sheet imaging. With a low-cost electrically tunable lens and minimum modification of an existing two-photon light-sheet imaging instrument, we demonstrated fast random depth hopping light-sheet imaging at 100 frames per second in the live brain slice. Through depth random-access, calcium activities for an astrocyte were recorded on four user-selected detection planes at a refreshing rate of 25 Hz.

Original languageEnglish (US)
Pages (from-to)26396-26406
Number of pages11
JournalOptics Express
Issue number15
StatePublished - Jul 18 2022

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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