Dendrimer type bio-reducible polymer for efficient gene delivery

Hye Yeong Nam; Kihoon Nam; Minhyung Lee; Sung Wan Kim; David A. Bull

doi:10.1016/j.jconrel.2012.04.025

Dendrimer type bio-reducible polymer for efficient gene delivery

Hye Yeong Nam, Kihoon Nam, Minhyung Lee, Sung Wan Kim, David A. Bull

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.

Original language	English (US)
Pages (from-to)	592-600
Number of pages	9
Journal	Journal of Controlled Release
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/j.jconrel.2012.04.025
State	Published - Jun 28 2012
Externally published	Yes

Keywords

Bioreducible polymer
Cytotoxicity
Dendrimer
Gene delivery
Transfection

ASJC Scopus subject areas

Pharmaceutical Science

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10.1016/j.jconrel.2012.04.025

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title = "Dendrimer type bio-reducible polymer for efficient gene delivery",

abstract = "Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.",

keywords = "Bioreducible polymer, Cytotoxicity, Dendrimer, Gene delivery, Transfection",

author = "Nam, {Hye Yeong} and Kihoon Nam and Minhyung Lee and Kim, {Sung Wan} and Bull, {David A.}",

note = "Funding Information: This work was financially supported by NIH grants HL 065477 (SWK) and HL 071541 (DAB). ",

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doi = "10.1016/j.jconrel.2012.04.025",

language = "English (US)",

volume = "160",

pages = "592--600",

journal = "Journal of Controlled Release",

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publisher = "Elsevier B.V.",

number = "3",

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T1 - Dendrimer type bio-reducible polymer for efficient gene delivery

AU - Nam, Hye Yeong

AU - Nam, Kihoon

AU - Lee, Minhyung

AU - Kim, Sung Wan

AU - Bull, David A.

N1 - Funding Information: This work was financially supported by NIH grants HL 065477 (SWK) and HL 071541 (DAB).

PY - 2012/6/28

Y1 - 2012/6/28

N2 - Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.

AB - Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.

KW - Bioreducible polymer

KW - Cytotoxicity

KW - Dendrimer

KW - Gene delivery

KW - Transfection

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M3 - Article

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AN - SCOPUS:84861982162

SN - 0168-3659

VL - 160

SP - 592

EP - 600

JO - Journal of Controlled Release

JF - Journal of Controlled Release

IS - 3

ER -

Dendrimer type bio-reducible polymer for efficient gene delivery

Abstract

Keywords

ASJC Scopus subject areas

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