Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

Alden K. Henderson; Henry I. Yamamura; Carlo A. Maggi; Stephen H. Buck; Paul L.M. Van Giersbergen; William R. Roeske

doi:10.1016/0922-4106(92)90099-H

Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

Alden K. Henderson, Henry I. Yamamura, Carlo A. Maggi, Stephen H. Buck, Paul L.M. Van Giersbergen, William R. Roeske

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In competitive radioligand binding assays, the NK₂ receptor antagonists [Tyr⁵,D-Trp^6,8,9,Arg¹⁰]NKA(4-10) (MEN 10207) and [Tyr⁵,D-Trp^6,8,9,Arg¹⁰]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK₂ receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK₂ receptor subtype.

Original language	English (US)
Pages (from-to)	175-178
Number of pages	4
Journal	European Journal of Pharmacology: Molecular Pharmacology
Volume	225
Issue number	2
DOIs	https://doi.org/10.1016/0922-4106(92)90099-H
State	Published - Feb 13 1992

Keywords

MEN 10207
MEN 10208
NK receptor subtype
Neurokinin A (NKA)

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0922-4106(92)90099-H

Cite this

@article{841990f9e4db41ea880fcc3ca8032da1,

title = "Demonstration of a neurokinin A receptor subtype in transfected fibroblasts",

abstract = "In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.",

keywords = "MEN 10207, MEN 10208, NK receptor subtype, Neurokinin A (NKA)",

author = "Henderson, {Alden K.} and Yamamura, {Henry I.} and Maggi, {Carlo A.} and Buck, {Stephen H.} and {Van Giersbergen}, {Paul L.M.} and Roeske, {William R.}",

note = "Funding Information: The authors thank Ms. Carol Haussler and Mina Reeder for their technical expertise with tissue cultures and Ms. Sue Waite for her comments on the manuscript. This work was supported by grants HL-20984 and DK-36289.",

year = "1992",

month = feb,

day = "13",

doi = "10.1016/0922-4106(92)90099-H",

language = "English (US)",

volume = "225",

pages = "175--178",

journal = "European Journal of Pharmacology: Molecular Pharmacology",

issn = "0922-4106",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

AU - Henderson, Alden K.

AU - Yamamura, Henry I.

AU - Maggi, Carlo A.

AU - Buck, Stephen H.

AU - Van Giersbergen, Paul L.M.

AU - Roeske, William R.

N1 - Funding Information: The authors thank Ms. Carol Haussler and Mina Reeder for their technical expertise with tissue cultures and Ms. Sue Waite for her comments on the manuscript. This work was supported by grants HL-20984 and DK-36289.

PY - 1992/2/13

Y1 - 1992/2/13

N2 - In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.

AB - In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.

KW - MEN 10207

KW - MEN 10208

KW - NK receptor subtype

KW - Neurokinin A (NKA)

UR - http://www.scopus.com/inward/record.url?scp=0026499990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026499990&partnerID=8YFLogxK

U2 - 10.1016/0922-4106(92)90099-H

DO - 10.1016/0922-4106(92)90099-H

M3 - Article

C2 - 1312943

AN - SCOPUS:0026499990

SN - 0922-4106

VL - 225

SP - 175

EP - 178

JO - European Journal of Pharmacology: Molecular Pharmacology

JF - European Journal of Pharmacology: Molecular Pharmacology

IS - 2

ER -

Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this