Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus

U. Hornemann; M. K. Speedie; L. H. Hurley; H. G. Floss

doi:10.1016/0006-291X(70)90245-7

Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus

U. Hornemann, M. K. Speedie, L. H. Hurley, H. G. Floss

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-¹⁴C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.

Original language	English (US)
Pages (from-to)	594-599
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(70)90245-7
State	Published - May 22 1970
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(70)90245-7

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@article{c2dd4824bf134529ab2833b9d0cc053d,

title = "Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus",

abstract = "A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.",

author = "U. Hornemann and Speedie, {M. K.} and Hurley, {L. H.} and Floss, {H. G.}",

note = "Funding Information: We thank Chas. Pfizer and Co. for providing us with an indolmycin-producing strain of 2. griseus and with a sample of indolmycin. We are also indebted Dr. H. R. Synder, University of Illinois, for a gift of B-methyltryptophan A and B. This work was supported by U.S. Public Health Service Grant No.",

year = "1970",

month = may,

day = "22",

doi = "10.1016/0006-291X(70)90245-7",

language = "English (US)",

volume = "39",

pages = "594--599",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "4",

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TY - JOUR

T1 - Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus

AU - Hornemann, U.

AU - Speedie, M. K.

AU - Hurley, L. H.

AU - Floss, H. G.

N1 - Funding Information: We thank Chas. Pfizer and Co. for providing us with an indolmycin-producing strain of 2. griseus and with a sample of indolmycin. We are also indebted Dr. H. R. Synder, University of Illinois, for a gift of B-methyltryptophan A and B. This work was supported by U.S. Public Health Service Grant No.

PY - 1970/5/22

Y1 - 1970/5/22

N2 - A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.

AB - A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.

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U2 - 10.1016/0006-291X(70)90245-7

DO - 10.1016/0006-291X(70)90245-7

M3 - Article

C2 - 5490210

AN - SCOPUS:0014958482

SN - 0006-291X

VL - 39

SP - 594

EP - 599

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomycesgriseus

Abstract

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