Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector

Jennifer L. Bromberg-White; Craig P. Webb; Veronique S. Patacsil; Cindy K. Miranti; Bart O. Williams; Sheri L. Holmen

doi:10.1128/JVI.78.9.4914-4916.2004

Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector

Jennifer L. Bromberg-White, Craig P. Webb, Veronique S. Patacsil, Cindy K. Miranti, Bart O. Williams, Sheri L. Holmen

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

Original language	English (US)
Pages (from-to)	4914-4916
Number of pages	3
Journal	Journal of virology
Volume	78
Issue number	9
DOIs	https://doi.org/10.1128/JVI.78.9.4914-4916.2004
State	Published - May 2004
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.78.9.4914-4916.2004

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title = "Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector",

abstract = "While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.",

author = "Bromberg-White, {Jennifer L.} and Webb, {Craig P.} and Patacsil, {Veronique S.} and Miranti, {Cindy K.} and Williams, {Bart O.} and Holmen, {Sheri L.}",

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AU - Bromberg-White, Jennifer L.

AU - Webb, Craig P.

AU - Patacsil, Veronique S.

AU - Miranti, Cindy K.

AU - Williams, Bart O.

AU - Holmen, Sheri L.

PY - 2004/5

Y1 - 2004/5

N2 - While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

AB - While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

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Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector

Abstract

ASJC Scopus subject areas

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