Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

Kazutaka Sumita; Hirofumi Yoshino; Mika Sasaki; Nazanin Majd; Emily Rose Kahoud; Hidenori Takahashi; Koh Takeuchi; Taruho Kuroda; Susan Lee; Pascale G. Charest; Kosuke Takeda; John M. Asara; Richard A. Firtel; Dimitrios Anastasiou; Atsuo T. Sasaki

doi:10.1074/jbc.M113.531178

Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

Kazutaka Sumita
, Hirofumi Yoshino
, Mika Sasaki
, Nazanin Majd
, Emily Rose Kahoud
, Hidenori Takahashi
, Koh Takeuchi
, Taruho Kuroda
, Susan Lee
, Pascale G. Charest
, Kosuke Takeda
, John M. Asara
, Richard A. Firtel
, Dimitrios Anastasiou
, Atsuo T. Sasaki

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

Original language	English (US)
Pages (from-to)	3950-3959
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M113.531178
State	Published - Feb 14 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Sumita, K., Yoshino, H., Sasaki, M., Majd, N., Kahoud, E. R., Takahashi, H., Takeuchi, K., Kuroda, T., Lee, S., Charest, P. G., Takeda, K., Asara, J. M., Firtel, R. A., Anastasiou, D., & Sasaki, A. T. (2014). Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis. Journal of Biological Chemistry, 289(7), 3950-3959. https://doi.org/10.1074/jbc.M113.531178

Sumita, K, Yoshino, H, Sasaki, M, Majd, N, Kahoud, ER, Takahashi, H, Takeuchi, K, Kuroda, T, Lee, S, Charest, PG, Takeda, K, Asara, JM, Firtel, RA, Anastasiou, D & Sasaki, AT 2014, 'Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis', Journal of Biological Chemistry, vol. 289, no. 7, pp. 3950-3959. https://doi.org/10.1074/jbc.M113.531178

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title = "Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis",

abstract = "Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.",

author = "Kazutaka Sumita and Hirofumi Yoshino and Mika Sasaki and Nazanin Majd and Kahoud, \{Emily Rose\} and Hidenori Takahashi and Koh Takeuchi and Taruho Kuroda and Susan Lee and Charest, \{Pascale G.\} and Kosuke Takeda and Asara, \{John M.\} and Firtel, \{Richard A.\} and Dimitrios Anastasiou and Sasaki, \{Atsuo T.\}",

year = "2014",

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day = "14",

doi = "10.1074/jbc.M113.531178",

language = "English (US)",

volume = "289",

pages = "3950--3959",

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T1 - Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

AU - Sumita, Kazutaka

AU - Yoshino, Hirofumi

AU - Sasaki, Mika

AU - Majd, Nazanin

AU - Kahoud, Emily Rose

AU - Takahashi, Hidenori

AU - Takeuchi, Koh

AU - Kuroda, Taruho

AU - Lee, Susan

AU - Charest, Pascale G.

AU - Takeda, Kosuke

AU - Asara, John M.

AU - Firtel, Richard A.

AU - Anastasiou, Dimitrios

AU - Sasaki, Atsuo T.

PY - 2014/2/14

Y1 - 2014/2/14

N2 - Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

AB - Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

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DO - 10.1074/jbc.M113.531178

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SN - 0021-9258

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 7

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Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

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