Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

Kazutaka Sumita; Hirofumi Yoshino; Mika Sasaki; Nazanin Majd; Emily Rose Kahoud; Hidenori Takahashi; Koh Takeuchi; Taruho Kuroda; Susan Lee; Pascale G. Charest; Kosuke Takeda; John M. Asara; Richard A. Firtel; Dimitrios Anastasiou; Atsuo T. Sasaki

doi:10.1074/jbc.M113.531178

Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

Kazutaka Sumita, Hirofumi Yoshino, Mika Sasaki, Nazanin Majd, Emily Rose Kahoud, Hidenori Takahashi, Koh Takeuchi, Taruho Kuroda, Susan Lee, Pascale G. Charest, Kosuke Takeda, John M. Asara, Richard A. Firtel, Dimitrios Anastasiou, Atsuo T. Sasaki

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

Original language	English (US)
Pages (from-to)	3950-3959
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M113.531178
State	Published - Feb 14 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Sumita, K., Yoshino, H., Sasaki, M., Majd, N., Kahoud, E. R., Takahashi, H., Takeuchi, K., Kuroda, T., Lee, S., Charest, P. G., Takeda, K., Asara, J. M., Firtel, R. A., Anastasiou, D., & Sasaki, A. T. (2014). Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis. Journal of Biological Chemistry, 289(7), 3950-3959. https://doi.org/10.1074/jbc.M113.531178

Sumita, K, Yoshino, H, Sasaki, M, Majd, N, Kahoud, ER, Takahashi, H, Takeuchi, K, Kuroda, T, Lee, S, Charest, PG, Takeda, K, Asara, JM, Firtel, RA, Anastasiou, D & Sasaki, AT 2014, 'Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis', Journal of Biological Chemistry, vol. 289, no. 7, pp. 3950-3959. https://doi.org/10.1074/jbc.M113.531178

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title = "Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis",

abstract = "Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.",

author = "Kazutaka Sumita and Hirofumi Yoshino and Mika Sasaki and Nazanin Majd and Kahoud, {Emily Rose} and Hidenori Takahashi and Koh Takeuchi and Taruho Kuroda and Susan Lee and Charest, {Pascale G.} and Kosuke Takeda and Asara, {John M.} and Firtel, {Richard A.} and Dimitrios Anastasiou and Sasaki, {Atsuo T.}",

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doi = "10.1074/jbc.M113.531178",

language = "English (US)",

volume = "289",

pages = "3950--3959",

journal = "Journal of Biological Chemistry",

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T1 - Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

AU - Sumita, Kazutaka

AU - Yoshino, Hirofumi

AU - Sasaki, Mika

AU - Majd, Nazanin

AU - Kahoud, Emily Rose

AU - Takahashi, Hidenori

AU - Takeuchi, Koh

AU - Kuroda, Taruho

AU - Lee, Susan

AU - Charest, Pascale G.

AU - Takeda, Kosuke

AU - Asara, John M.

AU - Firtel, Richard A.

AU - Anastasiou, Dimitrios

AU - Sasaki, Atsuo T.

PY - 2014/2/14

Y1 - 2014/2/14

N2 - Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

AB - Background: Targeting oncogenic K-Ras for cancer therapy has remained challenging. Results: Ubiquitination specifically occurs on the activated K-Ras orthologue in Dictyostelium via evolutionary conserved K-Ras lysines, which promotes K-Ras protein degradation. Conclusion: Our results indicate the existence of GTP-loaded K-Ras orthologue-specific degradation system in Dictyostelium. Significance: This work reveals a novel negative feedback regulation for the K-Ras isoform, which is critical for cytokinesis in Dictyostelium.

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Degradation of activated K-ras orthologue via K-ras-specific lysine residues is required for cytokinesis

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