@article{473262ea44a74aa6875648e8ad09cd17,
title = "Decrypting bacterial polyphenol metabolism in an anoxic wetland soil",
abstract = "Microorganisms play vital roles in modulating organic matter decomposition and nutrient cycling in soil ecosystems. The enzyme latch paradigm posits microbial degradation of polyphenols is hindered in anoxic peat leading to polyphenol accumulation, and consequently diminished microbial activity. This model assumes that polyphenols are microbially unavailable under anoxia, a supposition that has not been thoroughly investigated in any soil type. Here, we use anoxic soil reactors amended with and without a chemically defined polyphenol to test this hypothesis, employing metabolomics and genome-resolved metaproteomics to interrogate soil microbial polyphenol metabolism. Challenging the idea that polyphenols are not bioavailable under anoxia, we provide metabolite evidence that polyphenols are depolymerized, resulting in monomer accumulation, followed by the generation of small phenolic degradation products. Further, we show that soil microbiome function is maintained, and possibly enhanced, with polyphenol addition. In summary, this study provides chemical and enzymatic evidence that some soil microbiota can degrade polyphenols under anoxia and subvert the assumed polyphenol lock on soil microbial metabolism.",
author = "McGivern, {Bridget B.} and Tfaily, {Malak M.} and Borton, {Mikayla A.} and Kosina, {Suzanne M.} and Daly, {Rebecca A.} and Nicora, {Carrie D.} and Purvine, {Samuel O.} and Wong, {Allison R.} and Lipton, {Mary S.} and Hoyt, {David W.} and Northen, {Trent R.} and Hagerman, {Ann E.} and Wrighton, {Kelly C.}",
note = "Funding Information: B.B.M, A.E.H, K.C.W, T.R.N, and S.M.K were fully or partially supported by an Early Career Award to K.C.W from the National Science Foundation under Award Number 1750189. The soil sample collection and contributions by R.A.D were supported by an Early Career Award from the U.S. Department of Energy to K.C.W, Office of Science, Office of Biological and Environmental Research under Award Number DE-SC0019746. A portion of this research was performed under the Facilities Integrating Collaborations for User Science (FICUS) exploratory effort and used resources at the US Department of Energy (DOE) Joint Genome Institute (proposal ID 503154) and the Environmental Molecular Sciences Laboratory (proposal ID 49495), which are DOE Office of Science User Facilities. A portion of the research has been performed using EMSL (grid.436923.9), a DOE Office of Science User Facility sponsored by the Office of Biological and Environmental Research. A portion of the work has been performed at Lawrence Berkeley National Laboratory with use of resources at the US DOE Joint Genome Institute (proposal ID 2049) and the National Energy Research Scientific Computing Center, DOE Office of Science User Facilities with support by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research, Genomic Sciences Program under contract number DE-AC02-05CH11231 to Lawrence Berkeley National Laboratory. The authors wish to thank Ann Hess and the Franklin A. Graybill Statistics and Data Science Laboratory at Colorado State University for statistical consulting, and Elizabeth Eder for NMR technical analysis. The authors also wish to thank Wayne Zeller for running NMR characterization of the Sorghum CT polymer. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
day = "1",
doi = "10.1038/s41467-021-22765-1",
language = "English (US)",
volume = "12",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Research",
number = "1",
}