TY - JOUR
T1 - Decreased transcription of the sodium-phosphate transporter gene in the hypophosphatemic mouse
AU - Collins, J. F.
AU - Scheving, L. A.
AU - Ghishan, F. K.
PY - 1995
Y1 - 1995
N2 - Recently, it has been hypothesized that the proximal tubular Na+-P(i) transporter may play a role in murine X-linked hypophosphatemic vitamin D- resistant rickets. In the present investigation, Western blot analysis of renal brush-border membrane proteins, utilizing polyclonal antisera raised against the mouse Na+-P(i) transporter, revealed a predominant band at 87 kDa in normal and hypophosphatemic (Hyp) mice. The intensity of this band was reduced in the Hyp mouse by 4.5-fold (Hyp/normal = 0.22 ± 0.04, n = 3, P < 0.05). Additionally, immunohistochemical analysis of kidney cortex in both mice localized the protein to the apical membrane of the proximal tubules. Relative transcription rates of the Na+-P(i) transporter gene in the normal and Hyp mouse were then investigated. Nuclear run-on assays showed a 51 ± 0.02% decreased rate of transcription of the Na+-P(i) transporter gene in the Hyp mice (n = 3). Thus abnormal transcriptional control of this gene in the Hyp mouse likely plays a role in X-linked hypophosphatemia.
AB - Recently, it has been hypothesized that the proximal tubular Na+-P(i) transporter may play a role in murine X-linked hypophosphatemic vitamin D- resistant rickets. In the present investigation, Western blot analysis of renal brush-border membrane proteins, utilizing polyclonal antisera raised against the mouse Na+-P(i) transporter, revealed a predominant band at 87 kDa in normal and hypophosphatemic (Hyp) mice. The intensity of this band was reduced in the Hyp mouse by 4.5-fold (Hyp/normal = 0.22 ± 0.04, n = 3, P < 0.05). Additionally, immunohistochemical analysis of kidney cortex in both mice localized the protein to the apical membrane of the proximal tubules. Relative transcription rates of the Na+-P(i) transporter gene in the normal and Hyp mouse were then investigated. Nuclear run-on assays showed a 51 ± 0.02% decreased rate of transcription of the Na+-P(i) transporter gene in the Hyp mice (n = 3). Thus abnormal transcriptional control of this gene in the Hyp mouse likely plays a role in X-linked hypophosphatemia.
KW - Hyp mouse model
KW - X-linked hypophosphatemia
KW - transcription rate analysis
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U2 - 10.1152/ajprenal.1995.269.3.f439
DO - 10.1152/ajprenal.1995.269.3.f439
M3 - Article
C2 - 7573493
AN - SCOPUS:0029100530
SN - 1931-857X
VL - 269
SP - F439-F448
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 3 38-3
ER -