Abstract
Recently, it has been hypothesized that the proximal tubular Na+-Pi transporter may play a role in murine X-linked hypophosphatemic vitamin D-resistant rickets. In the present investigation, Western blot analysis of renal brush-border membrane proteins, utilizing polyclonal antisera raised against the mouse Na+-Pi transporter, revealed a predominant band at 87 kDa in normal and hypophosphatemic (Hyp) mice. The intensity of this band was reduced in the Hyp mouse by 4.5-fold (Hyp/normal = 0.22 ± 0.04, n = 3, P < 0.05). Additionally, immunohistochemical analysis of kidney cortex in both mice localized the protein to the apical membrane of the proximal tubules. Relative transcription rates of the Na+-Pi transporter gene in the normal and Hyp mouse were then investigated. Nuclear run-on assays showed a 51 ± 0.02% decreased rate of transcription of the Na+-Pi transporter gene in the Hyp mice (n = 3). Thus abnormal transcriptional control of this gene in the Hyp mouse likely plays a role in X-linked hypophosphatemia.
Original language | English (US) |
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Pages (from-to) | F439-F448 |
Journal | American Journal of Physiology - Renal Fluid and Electrolyte Physiology |
Volume | 269 |
Issue number | 3 38-3 |
State | Published - Sep 1995 |
Keywords
- Hyp mouse model
- Transcription rate analysis
- X-linked hypophosphatemia
ASJC Scopus subject areas
- Physiology