TY - GEN
T1 - Decreased Platelet Deposition in SIS-Based Vascular Grafts via Covalent Conjugation of RAFT Polymers
AU - Valencia-Rivero, Karen T.
AU - Cruz, Juan C.
AU - Briceno, Juan C.
AU - D'Amore, Antonio
AU - Ye, Sang Ho
AU - Geest, Jonathan Vande
AU - Wagner, William R.
N1 - Publisher Copyright:
© 2018 IEEE.
PY - 2018/9/18
Y1 - 2018/9/18
N2 - The marked tendency to promote thrombogenesis is a major obstacle for the implementation at the clinical level, of regenerative vascular grafts derived from extracellular matrices such as the small intestinal submucosa (SIS). SIS has around 66% (w/w) content of the extremely thrombogenic type I Collagen, which is appropriate for regeneration purposes, but still problematic for cardiovascular applications. To overcome this major hurdle, we covalently conjugated addition-fragmentation chain-transfer (RAFT) polymerized macromolecules of Zwitterionic Phosphorylcholine (PC) on dried SIS tubes previously formed from decellularized wet sheets. Our results indicate that even after a low covalent conjugation yield via 1-Ethyl-3-(3dimethylaminopropyl) carbodiimide (EDC), platelet deposition decreased by about 94% with respect to the unmodified surface. This was also the case when compared with the commercially available vascular graft material expanded polytetrafluoroethylene (ePTFE), where platelet deposition decreased by nearly 93% Thermal stability of modified SIS as well as regenerative and proliferation abilities remained unchanged as evidenced by constant melting temperatures and high growth levels of HUVEC cells after 3 and 8 days. Low cytotoxic levels were detected, and mechanical properties were improved, resembling the mechanical behavior of the native carotid artery. The combination of attributes of the modified material make it attractive for the next generation of regenerative vascular grafts.
AB - The marked tendency to promote thrombogenesis is a major obstacle for the implementation at the clinical level, of regenerative vascular grafts derived from extracellular matrices such as the small intestinal submucosa (SIS). SIS has around 66% (w/w) content of the extremely thrombogenic type I Collagen, which is appropriate for regeneration purposes, but still problematic for cardiovascular applications. To overcome this major hurdle, we covalently conjugated addition-fragmentation chain-transfer (RAFT) polymerized macromolecules of Zwitterionic Phosphorylcholine (PC) on dried SIS tubes previously formed from decellularized wet sheets. Our results indicate that even after a low covalent conjugation yield via 1-Ethyl-3-(3dimethylaminopropyl) carbodiimide (EDC), platelet deposition decreased by about 94% with respect to the unmodified surface. This was also the case when compared with the commercially available vascular graft material expanded polytetrafluoroethylene (ePTFE), where platelet deposition decreased by nearly 93% Thermal stability of modified SIS as well as regenerative and proliferation abilities remained unchanged as evidenced by constant melting temperatures and high growth levels of HUVEC cells after 3 and 8 days. Low cytotoxic levels were detected, and mechanical properties were improved, resembling the mechanical behavior of the native carotid artery. The combination of attributes of the modified material make it attractive for the next generation of regenerative vascular grafts.
KW - Biofunctionalization
KW - RAFT
KW - Reduced Platelet Deposition.
KW - Small Intestinal Submucosa
KW - Vascular Grafts
KW - Zwitterionic Macromolecules
UR - http://www.scopus.com/inward/record.url?scp=85055503926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055503926&partnerID=8YFLogxK
U2 - 10.1109/SIB.2018.8467727
DO - 10.1109/SIB.2018.8467727
M3 - Conference contribution
AN - SCOPUS:85055503926
T3 - 2018 9th International Seminar of Biomedical Engineering, SIB 2018 - Conference Proceedings
BT - 2018 9th International Seminar of Biomedical Engineering, SIB 2018 - Conference Proceedings
A2 - Nino, Ana Maria Rudas
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 9th International Seminar of Biomedical Engineering, SIB 2018
Y2 - 16 May 2018 through 18 May 2018
ER -