TY - JOUR
T1 - Dasheng
T2 - A recently amplified nonautonomous long terminal repeat element that is a major component of pericentromeric regions in rice
AU - Jiang, Ning
AU - Bao, Zhirong
AU - Temnykh, Svedana
AU - Cheng, Zhukuan
AU - Jiang, Jiming
AU - Wing, Rod A.
AU - McCouch, Susan R.
AU - Wessier, Susan R.
PY - 2002
Y1 - 2002
N2 - A new and unusual family of LTR elements, Dasheng, has been discovered in the genome of Oryza sativa following database searches of ∼100 Mb of rice genomic sequence and 78 Mb of BAC-end sequence information. With all of the cis-elements but none of the coding domains normally associated with retrotransposons (e.g., gag, pol), Dasheng is a novel nonautonomous LTR element with high copy number. Over half of the ∼1000 Dasheng elements in the rice genome are full length (5.6-8.6 kb), and 60% are estimated to have amplified in the past 500,000 years. Using a modified AFLP technique called transposon display, 215 elements were mapped to all 12 rice chromosomes. Interestingly, more than half of the mapped elements are clustered in the heterochromatic regions around centromeres. The distribution pattern was further confirmed by FISH analysis. Despite clustering in heterochromatin, Dasheng elements are not nested, suggesting their potential value as molecular markers for these marker-poor regions. Taken together, Dasheng is one of the highest-copy-number LTR elements and one of the most recent elements to amplify in the rice genome.
AB - A new and unusual family of LTR elements, Dasheng, has been discovered in the genome of Oryza sativa following database searches of ∼100 Mb of rice genomic sequence and 78 Mb of BAC-end sequence information. With all of the cis-elements but none of the coding domains normally associated with retrotransposons (e.g., gag, pol), Dasheng is a novel nonautonomous LTR element with high copy number. Over half of the ∼1000 Dasheng elements in the rice genome are full length (5.6-8.6 kb), and 60% are estimated to have amplified in the past 500,000 years. Using a modified AFLP technique called transposon display, 215 elements were mapped to all 12 rice chromosomes. Interestingly, more than half of the mapped elements are clustered in the heterochromatic regions around centromeres. The distribution pattern was further confirmed by FISH analysis. Despite clustering in heterochromatin, Dasheng elements are not nested, suggesting their potential value as molecular markers for these marker-poor regions. Taken together, Dasheng is one of the highest-copy-number LTR elements and one of the most recent elements to amplify in the rice genome.
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M3 - Article
C2 - 12136031
AN - SCOPUS:0036021406
SN - 0016-6731
VL - 161
SP - 1293
EP - 1305
JO - Genetics
JF - Genetics
IS - 3
ER -