TY - JOUR
T1 - Cytotoxic and other withanolides from aeroponically grown Physalis philadelphica
AU - Xu, Ya Ming
AU - Wijeratne, E. M.Kithsiri
AU - Brooks, Alan D.
AU - Tewary, Poonam
AU - Xuan, Li Jiang
AU - Wang, Wen Qiong
AU - Sayers, Thomas J.
AU - Gunatilaka, A. A.Leslie
N1 - Funding Information:
This work was supported by a grant from the Arizona Biomedical Research Commission [grant number ADHS-16-162515 ]. The project was also funded in whole or in part with federal funds from the gs2:National Cancer Institute, National Institutes of Health [contract number HHSN26120080001E] and [in part] by the Intramural Research Program of NIH , Frederick National Lab , Center for Cancer Research. We thank Mr. Daniel Bunting for his help with germination and aeroponic cultivation of P. philadelphica used in this work. Ashley Babyak and Hanna Marks are also thanked for their technical assistance in conducting cytotoxicity assays. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
Funding Information:
This work was supported by a grant from the Arizona Biomedical Research Commission [grant number ADHS-16-162515]. The project was also funded in whole or in part with federal funds from the gs2:National Cancer Institute, National Institutes of Health [contract number HHSN26120080001E] and [in part] by the Intramural Research Program of NIH, Frederick National Lab, Center for Cancer Research. We thank Mr. Daniel Bunting for his help with germination and aeroponic cultivation of P. philadelphica used in this work. Ashley Babyak and Hanna Marks are also thanked for their technical assistance in conducting cytotoxicity assays. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/8
Y1 - 2018/8
N2 - Eleven withanolides including six previously undescribed compounds, 16β-hydroxyixocarpanolide, 24,25-dihydroexodeconolide C, 16,17-dehydro-24-epi-dioscorolide A, 17-epi-philadelphicalactone A, 16-deoxyphiladelphicalactone C, and 4-deoxyixocarpalactone A were isolated from aeroponically grown Physalis philadelphica. Structures of these withanolides were elucidated by the analysis of their spectroscopic (HRMS, 1D and 2D NMR, ECD) data and comparison with published data for related withanolides. Cytotoxic activity of all isolated compounds was evaluated against a panel of five human tumor cell lines (LNCaP, ACHN, UO-31, M14 and SK-MEL-28), and normal (HFF) cells. Of these, 17-epi-philadelphicalactone A, withaphysacarpin, philadelphicalactone C, and ixocarpalactone A exhibited cytotoxicity against ACHN, UO-31, M14 and SK-MEL-28, but showed no toxicity to HFF cells.
AB - Eleven withanolides including six previously undescribed compounds, 16β-hydroxyixocarpanolide, 24,25-dihydroexodeconolide C, 16,17-dehydro-24-epi-dioscorolide A, 17-epi-philadelphicalactone A, 16-deoxyphiladelphicalactone C, and 4-deoxyixocarpalactone A were isolated from aeroponically grown Physalis philadelphica. Structures of these withanolides were elucidated by the analysis of their spectroscopic (HRMS, 1D and 2D NMR, ECD) data and comparison with published data for related withanolides. Cytotoxic activity of all isolated compounds was evaluated against a panel of five human tumor cell lines (LNCaP, ACHN, UO-31, M14 and SK-MEL-28), and normal (HFF) cells. Of these, 17-epi-philadelphicalactone A, withaphysacarpin, philadelphicalactone C, and ixocarpalactone A exhibited cytotoxicity against ACHN, UO-31, M14 and SK-MEL-28, but showed no toxicity to HFF cells.
KW - Aeroponic cultivation
KW - Chemical investigation
KW - Cytotoxic activity
KW - Physalis philadelphica
KW - Solanaceae
KW - Withanolides
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U2 - 10.1016/j.phytochem.2018.04.018
DO - 10.1016/j.phytochem.2018.04.018
M3 - Article
C2 - 29775868
AN - SCOPUS:85047088363
SN - 0031-9422
VL - 152
SP - 174
EP - 181
JO - Phytochemistry
JF - Phytochemistry
ER -