TY - JOUR
T1 - Cytochrome P450 and steroid hydroxylase activity in mouse olfactory and vomeronasal mucosa
AU - Gu, Jun
AU - Dudley, Carol
AU - Su, Ting
AU - Spink, David C.
AU - Zhang, Qing Yu
AU - Moss, Robert L.
AU - Ding, Xinxin
N1 - Funding Information:
We thank Dr. Charles B. Kasper of University of Wisconsin for the generous gift of anti-mEH antibody and Dr. Daniel W. Nebert of University of Cincinnati for providing the Cyp1a2(−/−) mice. The authors gratefully acknowledge the use of the Molecular Genetics Core of the Wadsworth Center. This investigation was supported in part by NIH Grant DC02640 (to X.D.) and DC02120 (to R.L.M.).
PY - 1999/12/9
Y1 - 1999/12/9
N2 - The aims of this study are to identify the sex steroid-metabolizing cytochrome P450 enzymes of the vomeronasal organ (VNO) and to determine the activities of VNO microsomes to metabolize estradiol, progesterone, and testosterone. Several P450 isoforms, including CYP1A2, CYP2A, CYP2B, CYP2C, CYP2G1, and CYP3A, NADPH P450-reductase, and microsomal epoxide hydrolase were detected in mouse VNO, although their expression levels were much lower than those in the main olfactory epithelium. VNO microsomes were active toward the three steroid hormones, producing metabolite profiles similar to those seen with olfactory mucosal microsomes. Thus, the mammalian VNO, a steroid hormone target tissue, contains multiple steroid-metabolizing P450 isoforms and is capable of metabolic disposition of the three major sex steroid hormones. These findings support the proposed roles of olfactory mucosal and VNO microsomal P450 enzymes in maintaining cellular hormonal homeostasis and other perireceptor processes associated with olfactory chemosensory function.
AB - The aims of this study are to identify the sex steroid-metabolizing cytochrome P450 enzymes of the vomeronasal organ (VNO) and to determine the activities of VNO microsomes to metabolize estradiol, progesterone, and testosterone. Several P450 isoforms, including CYP1A2, CYP2A, CYP2B, CYP2C, CYP2G1, and CYP3A, NADPH P450-reductase, and microsomal epoxide hydrolase were detected in mouse VNO, although their expression levels were much lower than those in the main olfactory epithelium. VNO microsomes were active toward the three steroid hormones, producing metabolite profiles similar to those seen with olfactory mucosal microsomes. Thus, the mammalian VNO, a steroid hormone target tissue, contains multiple steroid-metabolizing P450 isoforms and is capable of metabolic disposition of the three major sex steroid hormones. These findings support the proposed roles of olfactory mucosal and VNO microsomal P450 enzymes in maintaining cellular hormonal homeostasis and other perireceptor processes associated with olfactory chemosensory function.
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U2 - 10.1006/bbrc.1999.1807
DO - 10.1006/bbrc.1999.1807
M3 - Article
C2 - 10581200
AN - SCOPUS:0033539937
SN - 0006-291X
VL - 266
SP - 262
EP - 267
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -