Cystic fibrosis pigs develop lung disease and exhibit defective bacterial eradication at birth

David A. Stoltz, David K. Meyerholz, Alejandro A. Pezzulo, Shyam Ramachandran, Mark P. Rogan, Greg J. Davis, Robert A. Hanfland, Chris Wohlford-Lenane, Cassie L. Dohrn, Jennifer A. Bartlett, George A. Nelson IV, Chang Eugene, Peter J. Taft, Paula S. Ludwig, Mira Estin, Emma E. Hornick, Janice L. Launspach, Melissa Samuel, Tatiana Rokhlina, Philip H. KarpLynda S. Ostedgaard, Aliye Uc, Timothy D. Starner, Alexander R. Horswill, Kim A. Brogden, Randall S. Prather, Sandra S. Richter, Joel Shilyansky, Paul B. McCray, Joseph Zabner, Michael J. Welsh

Research output: Contribution to journalArticlepeer-review

391 Scopus citations


Lung disease causes most of the morbidity and mortality in cystic fibrosis (CF). Understanding the pathogenesis of this disease has been hindered, however, by the lack of an animal model with characteristic features of CF. To overcome this problem, we recently generated pigs with mutated CFTR genes. We now report that, within months of birth, CF pigs spontaneously developed hallmark features of CF lung disease, including airway inflammation, remodeling, mucus accumulation, and infection. Their lungs contained multiple bacterial species, suggesting that the lungs of CF pigs have a host defense defect against a wide spectrum of bacteria. In humans, the temporal and causal relations between inflammation and infection have remained uncertain. To investigate these processes, we studied newborn pigs. Their lungs showed no inflammation but were less often sterile than controls. Moreover, after introduction of bacteria into their lungs, pigs with CF failed to eradicate bacteria as effectively as wild-type pigs. These results suggest that impaired bacterial elimination is the pathogenic event that initiates a cascade of inflammation and pathology in CF lungs. Our finding that pigs with CF have a host defense defect against bacteria within hours of birth provides an opportunity to further investigate CF pathogenesis and to test therapeutic and preventive strategies that could be deployed before secondary consequences develop.

Original languageEnglish (US)
Pages (from-to)29ra31
JournalScience translational medicine
Issue number29
StatePublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Cystic fibrosis pigs develop lung disease and exhibit defective bacterial eradication at birth'. Together they form a unique fingerprint.

Cite this