Cultivated single-stranded DNA phages that infect marine bacteroidetes prove difficult to detect with DNA-binding stains

Karin Holmfeldt; Duško Odić; Matthew B. Sullivan; Mathias Middelboe; Lasse Riemann

doi:10.1128/AEM.06580-11

Cultivated single-stranded DNA phages that infect marine bacteroidetes prove difficult to detect with DNA-binding stains

Karin Holmfeldt
, Duško Odić
, Matthew B. Sullivan
, Mathias Middelboe
, Lasse Riemann

Ecology & Evolutionary Biology

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

Original language	English (US)
Pages (from-to)	892-894
Number of pages	3
Journal	Applied and environmental microbiology
Volume	78
Issue number	3
DOIs	https://doi.org/10.1128/AEM.06580-11
State	Published - Feb 2012

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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10.1128/AEM.06580-11

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abstract = "This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.",

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AU - Riemann, Lasse

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AB - This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

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Cultivated single-stranded DNA phages that infect marine bacteroidetes prove difficult to detect with DNA-binding stains

Abstract

ASJC Scopus subject areas

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