Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm

Zonghao Zeng, Lei Jin, Hongmin Li, Zhong Hu, Dacheng Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals of pokeweed antiviral protein (PAP) from seeds of Phytolacca americana with high diffraction ability were grown from high protein concentration (100 mg/mL) solution at high temperature (33°C). The crystal structure was solved by use of molecular replacement method and refined by use of molecular dynamic method at 0-25 nm to an R factor of 18.15% with standard deviations from standard geometry of 0.001 6 nm and 2.04° for bond lengths and bond angles, respectively. Comparison with two other PAPs revealed, near the active center, a sequence-and structure-variable region, consisting of the loop connecting the fifth β-strand with the second α-helix and including a proposed active residue, suggesting this loop probably to be related to difference in activity.

Original languageEnglish (US)
Pages (from-to)413-418
Number of pages6
JournalScience in China, Series C: Life Sciences
Volume41
Issue number4
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Crystal structure
  • Pokeweed antiviral protein
  • Ribosome inactivating protein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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