Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

C. Reed; G. D. Sturbaum; P. J. Hoover; C. R. Sterling

doi:10.1128/AEM.68.1.427-429.2002

Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

C. Reed, G. D. Sturbaum, P. J. Hoover, C. R. Sterling

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32 Scopus citations

Abstract

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

Original language	English (US)
Pages (from-to)	427-429
Number of pages	3
Journal	Applied and environmental microbiology
Volume	68
Issue number	1
DOIs	https://doi.org/10.1128/AEM.68.1.427-429.2002
State	Published - 2002

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/AEM.68.1.427-429.2002

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abstract = "Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.",

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Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

Abstract

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