Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis

Yong Zi Tan; José Rodrigues; James E. Keener; Ruixiang Blake Zheng; Richard Brunton; Brian Kloss; Sabrina I. Giacometti; Ana L. Rosário; Lei Zhang; Michael Niederweis; Oliver B. Clarke; Todd L. Lowary; Michael T. Marty; Margarida Archer; Clinton S. Potter; Bridget Carragher; Filippo Mancia

doi:10.1038/s41467-020-17202-8

Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis

Yong Zi Tan, José Rodrigues, James E. Keener, Ruixiang Blake Zheng, Richard Brunton, Brian Kloss, Sabrina I. Giacometti, Ana L. Rosário, Lei Zhang, Michael Niederweis, Oliver B. Clarke, Todd L. Lowary, Michael T. Marty, Margarida Archer, Clinton S. Potter, Bridget Carragher, Filippo Mancia

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.

Original language	English (US)
Article number	3396
Journal	Nature communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-17202-8
State	Published - Dec 1 2020

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-020-17202-8

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Tan, Y. Z., Rodrigues, J., Keener, J. E., Zheng, R. B., Brunton, R., Kloss, B., Giacometti, S. I., Rosário, A. L., Zhang, L., Niederweis, M., Clarke, O. B., Lowary, T. L., Marty, M. T., Archer, M., Potter, C. S., Carragher, B., & Mancia, F. (2020). Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis. Nature communications, 11(1), Article 3396. https://doi.org/10.1038/s41467-020-17202-8

Tan, YZ, Rodrigues, J, Keener, JE, Zheng, RB, Brunton, R, Kloss, B, Giacometti, SI, Rosário, AL, Zhang, L, Niederweis, M, Clarke, OB, Lowary, TL, Marty, MT, Archer, M, Potter, CS, Carragher, B & Mancia, F 2020, 'Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis', Nature communications, vol. 11, no. 1, 3396. https://doi.org/10.1038/s41467-020-17202-8

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abstract = "Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 {\AA} resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.",

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AU - Rodrigues, José

AU - Keener, James E.

AU - Zheng, Ruixiang Blake

AU - Brunton, Richard

AU - Kloss, Brian

AU - Giacometti, Sabrina I.

AU - Rosário, Ana L.

AU - Zhang, Lei

AU - Niederweis, Michael

AU - Clarke, Oliver B.

AU - Lowary, Todd L.

AU - Marty, Michael T.

AU - Archer, Margarida

AU - Potter, Clinton S.

AU - Carragher, Bridget

AU - Mancia, Filippo

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AB - Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.

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Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis

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Single-Particle Cryo-EM Structure of Arabinosyltransferase EmbB from Mycobacterium smegmatis

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Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis

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Single-Particle Cryo-EM Structure of Arabinosyltransferase EmbB from Mycobacterium smegmatis

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