Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

Lokenga Badinga; Robert J. Collier; William W. Thatcher; Sergio J. Quintana; Fuller W. Bazer

doi:10.1016/0303-7207(87)90100-6

Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

Lokenga Badinga
, Robert J. Collier
, William W. Thatcher
, Sergio J. Quintana
, Fuller W. Bazer

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([¹²⁵I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated M_r of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [¹²⁵I]rbGH into the M_r = 140000 species. In dairy bulls, the M_r = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of M_r = 119000 which does not recognize bPRL.

Original language	English (US)
Pages (from-to)	85-89
Number of pages	5
Journal	Molecular and Cellular Endocrinology
Volume	52
Issue number	1-2
DOIs	https://doi.org/10.1016/0303-7207(87)90100-6
State	Published - Jul 1987
Externally published	Yes

Keywords

Bovine growth hormone
Cross-linking
Somatotropin receptor

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(87)90100-6

Cite this

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title = "Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes",

abstract = "The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.",

keywords = "Bovine growth hormone, Cross-linking, Somatotropin receptor",

author = "Lokenga Badinga and Collier, \{Robert J.\} and Thatcher, \{William W.\} and Quintana, \{Sergio J.\} and Bazer, \{Fuller W.\}",

year = "1987",

month = jul,

doi = "10.1016/0303-7207(87)90100-6",

language = "English (US)",

volume = "52",

pages = "85--89",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

AU - Badinga, Lokenga

AU - Collier, Robert J.

AU - Thatcher, William W.

AU - Quintana, Sergio J.

AU - Bazer, Fuller W.

PY - 1987/7

Y1 - 1987/7

N2 - The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.

AB - The structure of bovine somatotropin receptor was examined following covalent coupling of iodinated recombinant bovine growth hormone ([125I]rbGH) to bovine liver membrane receptors using ethylene glycol bis(succinimidyl succinate). Iodinated rbGH was incorporated into a complex of estimated Mr of 140000 under reducing conditions. Excess unlabeled rbGH, but not bovine prolactin (bPRL), inhibited completely the incorporation of [125I]rbGH into the Mr = 140000 species. In dairy bulls, the Mr = 140000 complex was undetectable soon after birth but became predominant at 6 months of age. No evidence was found to support presence of bPRL receptors in steer liver membranes. Assuming a 1:1 stoichiometry of hormone binding to receptor, it appears that bGH binds to a major receptor subunit of Mr = 119000 which does not recognize bPRL.

KW - Bovine growth hormone

KW - Cross-linking

KW - Somatotropin receptor

UR - https://www.scopus.com/pages/publications/0023185045

UR - https://www.scopus.com/pages/publications/0023185045#tab=citedBy

U2 - 10.1016/0303-7207(87)90100-6

DO - 10.1016/0303-7207(87)90100-6

M3 - Article

C2 - 3622922

AN - SCOPUS:0023185045

SN - 0303-7207

VL - 52

SP - 85

EP - 89

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1-2

ER -

Covalent coupling of bovine growth hormone to its receptor in bovine liver membranes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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