@article{407b28946f7840e0b5de12941cd4f1c2,
title = "COP1, an arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain",
abstract = "Plant seedling development is capable of following 1 of 2 distinct morphogenic pathways: skotomorphogenesis in darkness and photomorphogenesis in light. Dark-grown Arabidopsis seedlings with recessive mutations at the constitutively photomorphogenic (COP1) locus indicate that the wild-type COP1 protein represses photomorphogenesis in darkness and that light reverses this repressive activity. Using a T-DNA-tagged mutant, we have cloned the COP1 locus. The amino-terminal half of the encoded protein contains a conserved zinc-binding motif, whereas the carboxylterminal half contains a domain homologous to the WD-40 repeat motif of Gβ proteins. The presence of both a putative DNA-binding motif and a G protein-related domain in a single polypeptide suggests that COP1 may be the first of a new class of regulatory molecules. This novel structure could endow COP1 with the capacity to function as a negative transcriptional regulator capable of direct interaction with components of the G protein signaling pathway.",
author = "Deng, {Xing Wang} and Minami Matsui and Ning Wei and Doris Wagner and Chu, {Angela M.} and Feldmann, {Kenneth A.} and Quail, {Peter H.}",
note = "Funding Information: We thank Tim Caspar and Judy Roe for valuable discussrons during the course of this work; Barry Piekos of the Biology Department electron microscope laboratory at Yale Universrty for technrcal assistance with the microscopy analysis; Katie Dehesh, Athanasios Theologis, Albrecht von Arnim, Sarah Hake, and Trmothy McNellis for comments on the manuscript; and Ron Wells for preparing and editing the manuscript. We also thank the Du Pont company for allowing us to screen their T-DNA-tagged Arabidopsis lines. This work was supported earlier by United States Department of Agriculture (USDA)IAgriculture Research Service CRIS #5335-23000-004-OOD (to P. H. Q.) and later by Natronal Institutes of Health grant l-R29-GM47850-01 (molecular analysis of COPl) and USDA grant 92-37301-7706 (scanning electron microscope analysis) (to X.-W. D.). X.-W. D. was a Du Pont Fellow of the Lrfe Sciences Research Foundation.",
year = "1992",
month = nov,
day = "27",
doi = "10.1016/0092-8674(92)90555-Q",
language = "English (US)",
volume = "71",
pages = "791--801",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}