Contributions of cis- And trans-Regulatory Evolution to Transcriptomic Divergence across Populations in the Drosophila mojavensis Larval Brain

Kyle M. Benowitz, Joshua M. Coleman, Carson W. Allan, Luciano M. Matzkin

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Natural selectionongene expressionwasoriginallypredictedto resultprimarily incis- rather thantrans-regulatory evolution,due tothe expectation of reduced pleiotropy. Despite this, numerous studies have ascribed recent evolutionary divergence in gene expression predominantly to trans-regulation. Performing RNA-seq on single isofemale lines from genetically distinct populations of the cactophilic flyDrosophilamojavensis and their F1 hybrids,werecapitulated this pattern in both larval brains andwhole bodies.However,we demonstrate that improving the measurement of brain expression divergence between populations by using seven additional genotypes considerably reduces the estimate of trans-regulatory contributions to expression evolution. We argue that the finding of trans-regulatory predominance can result frombiases due to environmental variation in expression or other sources of noise, and that cis-regulation is likely a greater contributor to transcriptional evolution across D.mojavensis populations. Lastly,wemerge these lines of data to identify several previously hypothesized and intriguing novel candidate genes, and suggest that the integration of regulatory and population-level transcriptomic data can provide useful filters for the identification of potentially adaptive genes.

Original languageEnglish (US)
Pages (from-to)1407-1418
Number of pages12
JournalGenome biology and evolution
Volume12
Issue number8
DOIs
StatePublished - 2020

Keywords

  • Cactophilic
  • Local adaptation
  • Pleiotropy
  • RNA-seq
  • Transcriptional regulation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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