@article{19b4736cb677414cb8b234d132c9ea8a,
title = "Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System",
abstract = "Microbial communities in incipient soil systems serve as the only biotic force shaping landscape evolution. However, the underlying ecological forces shaping microbial community structure and function are inadequately understood. We used amplicon sequencing to determine microbial taxonomic assembly and metagenome sequencing to evaluate microbial functional assembly in incipient basaltic soil subjected to precipitation. Community composition was stratified with soil depth in the pre-precipitation samples, with surficial communities maintaining their distinct structure and diversity after precipitation, while the deeper soil samples appeared to become more uniform. The structural community assembly remained deterministic in pre- and post-precipitation periods, with homogenous selection being dominant. Metagenome analysis revealed that carbon and nitrogen functional potential was assembled stochastically. Sub-populations putatively involved in the nitrogen cycle and carbon fixation experienced counteracting assembly pressures at the deepest depths, suggesting the communities may functionally assemble to respond to short-term environmental fluctuations and impact the landscape-scale response to perturbations. We propose that contrasting assembly forces impact microbial structure and potential function in an incipient landscape; in situ landscape characteristics (here homogenous parent material) drive community structure assembly, while short-term environmental fluctuations (here precipitation) shape environmental variations that are random in the soil depth profile and drive stochastic sub-population functional dynamics.",
keywords = "16S amplicon sequencing, community assembly, incipient soil, landscape evolution, metagenome",
author = "Aditi Sengupta and Volkmann, {Till H.M.} and Danczak, {Robert E.} and Stegen, {James C.} and Katerina Dontsova and Nate Abramson and Bugaj, {Aaron S.} and Volk, {Michael J.} and Matos, {Katarena A.} and Meira-Neto, {Antonio A.} and Albert Barber{\'a}n and Neilson, {Julia W.} and Maier, {Raina M.} and Jon Chorover and Troch, {Peter A.} and Meredith, {Laura K.}",
note = "Funding Information: AS was supported by Biosphere 2 through the office of the Senior Vice President for Research Innovation and Impact at the University of Arizona. The authors gratefully acknowledge financial support from the Philecology Foundation and support of NSF-funded projects EAR-1344552, EAR-1340912, EAR-141709, and OIA-2121134. AS would like to acknowledge the intellectual inspiration provided by Ahana Sen in drafting the final version of the manuscript. AM-N would like to acknowledge the support received by the Brazilian Scientific Mobility Program promoted by CAPES. Additional funding support were provided by the Water, Environmental, and Energy Solutions (WEES) Initiative at the University of Arizona and by the Office of Research, Discovery and Innovation{\textquoteright}s Accelerate for Success Grant at the University of Arizona. The authors would also like to acknowledge Daniel Laubitz at the University of Arizona Genomics Core for method development and sequencing of low-template samples. JS and RD were supported by the United States Department of Energy (DOE), Office of Biological and Environmental Research (BER), as part of Subsurface Biogeochemical Research Program{\textquoteright}s Scientific Focus Area (SFA) at Pacific Northwest National Laboratory (PNNL). PNNL is operated for DOE by Battelle Memorial Institute under contract DE-AC06-76RLO 1830. Metagenome sequencing was conducted at the United States Department of Energy Funding Information: AS was supported by Biosphere 2 through the office of the Senior Vice President for Research Innovation and Impact at the University of Arizona. The authors gratefully acknowledge financial support from the Philecology Foundation and support of NSF-funded projects EAR-1344552, EAR-1340912, EAR-141709, and OIA-2121134. AS would like to acknowledge the intellectual inspiration provided by Ahana Sen in drafting the final version of the manuscript. AM-N would like to acknowledge the support received by the Brazilian Scientific Mobility Program promoted by CAPES. Additional funding support were provided by the Water, Environmental, and Energy Solutions (WEES) Initiative at the University of Arizona and by the Office of Research, Discovery and Innovation{\textquoteright}s Accelerate for Success Grant at the University of Arizona. The authors would also like to acknowledge Daniel Laubitz at the University of Arizona Genomics Core for method development and sequencing of low-template samples. JS and RD were supported by the United States Department of Energy (DOE), Office of Biological and Environmental Research (BER), as part of Subsurface Biogeochemical Research Program{\textquoteright}s Scientific Focus Area (SFA) at Pacific Northwest National Laboratory (PNNL). PNNL is operated for DOE by Battelle Memorial Institute under contract DE-AC06-76RLO 1830. Metagenome sequencing was conducted at the United States Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, which is supported by the Office of Science of the United States Department of Energy under Contract No. DE-AC02-05CH11231 under Community Science Program Project 502880. Publisher Copyright: Copyright {\textcopyright} 2021 Sengupta, Volkmann, Danczak, Stegen, Dontsova, Abramson, Bugaj, Volk, Matos, Meira-Neto, Barber{\'a}n, Neilson, Maier, Chorover, Troch and Meredith.",
year = "2021",
month = nov,
day = "23",
doi = "10.3389/fmicb.2021.754698",
language = "English (US)",
volume = "12",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
}