TY - JOUR
T1 - Continuous separation of serum proteins using a stirred cell charged with carboxylated and sulfonated microspheres
AU - Lee, Jung Hun
AU - Yoon, Jeong Yeol
AU - Kim, Woo Sik
PY - 1998
Y1 - 1998
N2 - We contrived a new separation system using a stirred cell charged with uncoupled microsphere similar to the chromatographic separation. Microspheres, carboxylated PS/PMAA and sulfonated PS/PNaSS, were prepared by emulsifier-free emulsion polymerization. To complement the submicron size weakness and the absence of ligands, we employed the latex form, the dispersion of microsphere, and took advantage of interaction relationships between proteins and microspheres. Adsorption isotherm is contemplated to investigate continuous separation behaviours of serum proteins. Selectivity of separation is in the following order: PS/PNaSS-2.0 (high sulfonated) < PS/PNaSS-0.3 (low sulfonated) < PS/PMAA-0.5 (low carboxylated). Unlike previous works on batch separation, not only the adsorbed amount in equilibrium (C(m)), but also adsorption coefficient (K), played an important role in continuous separation. Functional groups (carboxyl and sulfonate), induced from the co-monomer, also affected the adsorption behaviours.
AB - We contrived a new separation system using a stirred cell charged with uncoupled microsphere similar to the chromatographic separation. Microspheres, carboxylated PS/PMAA and sulfonated PS/PNaSS, were prepared by emulsifier-free emulsion polymerization. To complement the submicron size weakness and the absence of ligands, we employed the latex form, the dispersion of microsphere, and took advantage of interaction relationships between proteins and microspheres. Adsorption isotherm is contemplated to investigate continuous separation behaviours of serum proteins. Selectivity of separation is in the following order: PS/PNaSS-2.0 (high sulfonated) < PS/PNaSS-0.3 (low sulfonated) < PS/PMAA-0.5 (low carboxylated). Unlike previous works on batch separation, not only the adsorbed amount in equilibrium (C(m)), but also adsorption coefficient (K), played an important role in continuous separation. Functional groups (carboxyl and sulfonate), induced from the co-monomer, also affected the adsorption behaviours.
UR - http://www.scopus.com/inward/record.url?scp=0031742622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031742622&partnerID=8YFLogxK
U2 - 10.1002/(sici)1099-0801(199811/12)12:6<330::aid-bmc756>3.0.co;2-%23
DO - 10.1002/(sici)1099-0801(199811/12)12:6<330::aid-bmc756>3.0.co;2-%23
M3 - Article
C2 - 9861492
AN - SCOPUS:0031742622
SN - 0269-3879
VL - 12
SP - 330
EP - 334
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 6
ER -