Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi; Robert A. Creelman; John E. Mullet; Rod A. Wing

doi:10.1007/BF02668782

Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi
, Robert A. Creelman
, John E. Mullet
, Rod A. Wing

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

Original language	English (US)
Pages (from-to)	124-128
Number of pages	5
Journal	Plant Molecular Biology Reporter
Volume	13
Issue number	2
DOIs	https://doi.org/10.1007/BF02668782
State	Published - Jun 1995
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02668782

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AU - Wing, Rod A.

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AB - We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

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Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Abstract

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