TY - JOUR
T1 - Conjugated equine estrogen treatment corrected the exacerbated aorta oxidative stress in ovariectomized spontaneously hypertensive rats
AU - Ceravolo, Graziela S.
AU - Filgueira, Fernando P.
AU - Costa, Tiago J.
AU - Lobato, Nubia S.
AU - Chignalia, Andréia Z.
AU - Araujo, Priscila X.
AU - Tostes, Rita C.
AU - Dantas, Ana P.
AU - Fortes, Zuleica B.
AU - Carvalho, Maria Helena C.
N1 - Funding Information:
The authors are thankful to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Pesquisa (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil and Direção Geral de Universidades, Spain (CAPES/DGU 269/12). G.S.C. was supported by a post-doctoral fellowship from FAPESP (07-59278-7).
PY - 2013/3
Y1 - 2013/3
N2 - Objective: The increased risk of cardiovascular diseases in postmenopausal women has been linked to the decrease in plasma estrogen levels. Preparation of conjugate equine estrogens (CEE) is one of the most routinely used hormone therapy in postmenopausal women. However, studies on the vascular effects of CEE are still sparse and the mechanism of action is not completely elucidated. In this context, we have determined the effects of CEE in the vascular oxidative stress observed in ovariectomyzed (OVX) spontaneously hypertensive rats (SHR). Mechanisms by which CEE interferes with redox-sensitive pathways and endothelial function were also determined. Results: Aortas from OVX rats exhibited increased generation of reactive oxygen species (ROS), NADPH oxidase activity and reduced catalase protein expression, compared to aortas from sham SHR. Endothelium-intact aortic rings from OVX were hyperreactive to NE when compared to Sham aortas. This hyperreactivity was corrected by superoxide dismutase (SOD), catalase, and endothelium removal. Treatment of OVX-SHR with CEE reduced vascular ROS generation, NADPH oxidase activity, enhanced SOD and catalase expression and also corrected the NE-hyperreactivity in aortic rings from OVX-SHR. Conclusion: Our study indicates a potential benefit of CEE therapy through a mechanism that involves reduction in oxidative stress, improving endothelial function in OVX hypertensive rats.
AB - Objective: The increased risk of cardiovascular diseases in postmenopausal women has been linked to the decrease in plasma estrogen levels. Preparation of conjugate equine estrogens (CEE) is one of the most routinely used hormone therapy in postmenopausal women. However, studies on the vascular effects of CEE are still sparse and the mechanism of action is not completely elucidated. In this context, we have determined the effects of CEE in the vascular oxidative stress observed in ovariectomyzed (OVX) spontaneously hypertensive rats (SHR). Mechanisms by which CEE interferes with redox-sensitive pathways and endothelial function were also determined. Results: Aortas from OVX rats exhibited increased generation of reactive oxygen species (ROS), NADPH oxidase activity and reduced catalase protein expression, compared to aortas from sham SHR. Endothelium-intact aortic rings from OVX were hyperreactive to NE when compared to Sham aortas. This hyperreactivity was corrected by superoxide dismutase (SOD), catalase, and endothelium removal. Treatment of OVX-SHR with CEE reduced vascular ROS generation, NADPH oxidase activity, enhanced SOD and catalase expression and also corrected the NE-hyperreactivity in aortic rings from OVX-SHR. Conclusion: Our study indicates a potential benefit of CEE therapy through a mechanism that involves reduction in oxidative stress, improving endothelial function in OVX hypertensive rats.
KW - Conjugated equine estrogens
KW - Hypertension
KW - Ovariectomyzed rats
KW - Vascular reactivity
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U2 - 10.1016/j.steroids.2012.11.018
DO - 10.1016/j.steroids.2012.11.018
M3 - Article
C2 - 23261957
AN - SCOPUS:84873034344
SN - 0039-128X
VL - 78
SP - 341
EP - 346
JO - Steroids
JF - Steroids
IS - 3
ER -