TY - JOUR
T1 - Conformational study of a somatostatin analogue by high‐field n.m.r. spectroscopy, in aqueous solution
AU - WYNANTS, C.
AU - SUGG, E.
AU - HRUBY, V. J.
AU - VAN BINST, G.
PY - 1987/10
Y1 - 1987/10
N2 - The cyclic analogue of somatostatin (SRIF), D‐Phe‐Cys‐Tyr‐D‐Trp‐Lys‐Thr‐Cys‐Thr‐NH2 (CTC), exhibits good affinity for both opioid and SRIF receptor systems. Its conformational properties were examined in water by high‐field proton n.m.r. spectroscopy and compared with results previously obtained with structurally related analogues SMS 201–995 and Sandoz 204–090 in the same solvent. The assignments were made using 2 D‐n.m.r. methods, especially long‐range connectivities between neighbouring α protons, and between β and aromatic protons. The 3JNH‐cαH and Δδ/ΔT values are compatible with an equilibrium between two γ turns involving residues 2, 3 and 4 and residues 3, 4, and 5, respectively.
AB - The cyclic analogue of somatostatin (SRIF), D‐Phe‐Cys‐Tyr‐D‐Trp‐Lys‐Thr‐Cys‐Thr‐NH2 (CTC), exhibits good affinity for both opioid and SRIF receptor systems. Its conformational properties were examined in water by high‐field proton n.m.r. spectroscopy and compared with results previously obtained with structurally related analogues SMS 201–995 and Sandoz 204–090 in the same solvent. The assignments were made using 2 D‐n.m.r. methods, especially long‐range connectivities between neighbouring α protons, and between β and aromatic protons. The 3JNH‐cαH and Δδ/ΔT values are compatible with an equilibrium between two γ turns involving residues 2, 3 and 4 and residues 3, 4, and 5, respectively.
KW - 1H‐n.m.r
KW - conformation
KW - somatostatin analogue
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U2 - 10.1111/j.1399-3011.1987.tb03363.x
DO - 10.1111/j.1399-3011.1987.tb03363.x
M3 - Article
C2 - 2892808
AN - SCOPUS:0023424011
SN - 0367-8377
VL - 30
SP - 541
EP - 547
JO - International journal of peptide and protein research
JF - International journal of peptide and protein research
IS - 4
ER -