TY - JOUR
T1 - Conformation of oxytocin studied by laser Raman spectroscopy
AU - Tu, Anthony T.
AU - Bjarnason, Jón B.
AU - Hruby, Victor J.
N1 - Funding Information:
sharp peak. Based on the previous study of Sugeta et al. \[25\], the conformation of C-C-S-S-C-C in the disulfide bridge of oxytocin in the ring is gauche-gauche-gauche. This investigation was supported by NIH grants 5 R01 GM 191172 (A.T.T.) and 5 R01 AM17420 and by the National Science Foundation (V.J.H.).
PY - 1978/4/26
Y1 - 1978/4/26
N2 - The peptide backbone conformation and salient structural details of oxytocin were examined by laser Raman spectroscopy. Spectra were obtained in the solid phase, water, 2H2O, and dimethyl sulfoxide solutions. A distinct Amide I band was obtained at 1663 cm-1 for aqueous and deuterated samples and 1666 cm-1 for the solid sample. A relatively high frequency Amide III band at 1260 cm-1 was obtained. It is concluded that these Amide I and III bands arise from the "β-turn"-like conformation of oxytocin. The tyrosine side chain, according to the I850 cm-1/I830cm-1 intensity ratio, is exposed to the solvent. The S-S stretching vibration at 512 cm-1 indicates the conformation of C-C-S-S-C-C in the disulfide bridge of oxytocin in the ring is gauche-gauche-gauche.
AB - The peptide backbone conformation and salient structural details of oxytocin were examined by laser Raman spectroscopy. Spectra were obtained in the solid phase, water, 2H2O, and dimethyl sulfoxide solutions. A distinct Amide I band was obtained at 1663 cm-1 for aqueous and deuterated samples and 1666 cm-1 for the solid sample. A relatively high frequency Amide III band at 1260 cm-1 was obtained. It is concluded that these Amide I and III bands arise from the "β-turn"-like conformation of oxytocin. The tyrosine side chain, according to the I850 cm-1/I830cm-1 intensity ratio, is exposed to the solvent. The S-S stretching vibration at 512 cm-1 indicates the conformation of C-C-S-S-C-C in the disulfide bridge of oxytocin in the ring is gauche-gauche-gauche.
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U2 - 10.1016/0005-2795(78)90399-9
DO - 10.1016/0005-2795(78)90399-9
M3 - Article
C2 - 647024
AN - SCOPUS:0017806391
SN - 1570-9639
VL - 533
SP - 530
EP - 533
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 2
ER -