Complex regulation of Ly-6E gene transcription in T cells by IFNs

Mehran M. Khodadoust, Khuda Dad Khan, Alfred L.M. Bothwell

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The complexity of IFN-mediated regulation of the murine Ly-6E gene in T cell lines is highlighted by the following observations: 1) multiple regulatory regions are present within different parts of the Ly-6E promoter and are necessary for IFN inducibility of the Ly-6E gene, 2) multiple transcription factors including Oct-1 and Oct-2 and the high mobility group (HMG) protein HMGI(Y) bind to regulatory elements present within the G region required for both IFN-αβ and IFN-γ responses, 3) mutational analysis of the G region reveals that a complex interaction exists between the factors binding to this region as shown by their mutual interdependence for detection in DMSA, and 4) inhibition of expression of HMG proteins by antisense HMGI-C RNA in EL4 cells causes the loss of IFN-αβ and IFN-γ inducibility of the endogenous Ly-6 gene. These findings taken together suggest that, in response to IFN treatment, an HMG protein-dependent complex involving multiple regulatory factors is assembled and is required for IFN inducibility of the Ly-6E gene.

Original languageEnglish (US)
Pages (from-to)811-819
Number of pages9
JournalJournal of Immunology
Volume163
Issue number2
StatePublished - Jul 15 1999
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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