TY - JOUR
T1 - Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination
AU - Ramos-Sobrinho, Roberto
AU - Ferro, Mayra M.M.
AU - Nagata, Tatsuya
AU - Puig, Alina S.
AU - Keith, Cory Von
AU - Britto, Dahyana Santos
AU - Gutierrez, Osman A.
AU - Marelli, Jean Philippe
AU - Brown, Judith K.
N1 - Funding Information:
Funding for this research was provided by MARS Inc. through Trust Agreement 6038-21000-023-12-T and by USDA-ARS, Miami under Agreement FAIN 58-6038-6-008.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2021/7
Y1 - 2021/7
N2 - To analyze the DNA virome associated with cacao (Theobroma cacao L.) trees showing virus-like symptoms in Brazil (BR) and Puerto Rico (PR) during 2018-2019, total DNA was isolated from symptomatic leaves and subjected to high-throughput Illumina sequencing. The assembled complete badnaviral genome sequences were verified by PCR amplification, cloning, and DNA sequencing. Based on pairwise distances and phylogenetic analysis, three badnaviral genomes were identified, and these viruses were found to be isolates of the previously described cacao mild mosaic virus (CaMMV). The three genomes were 7,520, 7,524, and 7,514 bp in size for the isolates CaMMV-BR321, CaMMV-BR322, and CaMMV-PR3, respectively. Each genome contained four predicted open reading frames: ORFs 1-3 and ORFY. The CaMMV-PR3 isolate was identified as a probable recombinant, with a CaMMV-BR-like virus as the major parent.
AB - To analyze the DNA virome associated with cacao (Theobroma cacao L.) trees showing virus-like symptoms in Brazil (BR) and Puerto Rico (PR) during 2018-2019, total DNA was isolated from symptomatic leaves and subjected to high-throughput Illumina sequencing. The assembled complete badnaviral genome sequences were verified by PCR amplification, cloning, and DNA sequencing. Based on pairwise distances and phylogenetic analysis, three badnaviral genomes were identified, and these viruses were found to be isolates of the previously described cacao mild mosaic virus (CaMMV). The three genomes were 7,520, 7,524, and 7,514 bp in size for the isolates CaMMV-BR321, CaMMV-BR322, and CaMMV-PR3, respectively. Each genome contained four predicted open reading frames: ORFs 1-3 and ORFY. The CaMMV-PR3 isolate was identified as a probable recombinant, with a CaMMV-BR-like virus as the major parent.
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U2 - 10.1007/s00705-021-05063-5
DO - 10.1007/s00705-021-05063-5
M3 - Article
C2 - 33900470
AN - SCOPUS:85105251007
SN - 0304-8608
VL - 166
SP - 2027
EP - 2031
JO - Archives of virology
JF - Archives of virology
IS - 7
ER -