Complementary functions of two recessive R-genes determine resistance durability of tobacco 'Virgin A Mutant' (VAM) to Potato virus Y

Rodolfo Acosta-Leal; Zhongguo Xiong

doi:10.1016/j.virol.2008.06.026

Complementary functions of two recessive R-genes determine resistance durability of tobacco 'Virgin A Mutant' (VAM) to Potato virus Y

Rodolfo Acosta-Leal, Zhongguo Xiong

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Tobaccos VAM and NC745 carry the recessive va gene that confers resistance to PVY_NN. However, they exhibit different levels of resistance durability. Upon virus inoculation, only NC745 developed sporadic systemic symptoms caused by emerging resistance-breaking variants that easily infected both NC745 and VAM genotypes. To identify the differential host conditions associated with this phenomenon, cellular accumulation, cell-to-cell movement, vascular translocation, and foliar content of PVY_NN were comparatively evaluated. Virus cell-to-cell movement was restricted and its transit through the vasculature boundaries was completely blocked in both tobacco varieties. However, an additional defense mechanism operating only in tobacco VAM drastically reduced the in situ cellular virus accumulation. Genetic analyses of hybrid plant progenies indicate that VAM-type resistance was conditioned by at least two recessive genes: va and a newly reported va2 locus. Moreover, segregant plant progenies that restricted virus movement but permitted normal virus accumulation were prone to develop resistance-breaking infections.

Original language	English (US)
Pages (from-to)	275-283
Number of pages	9
Journal	Virology
Volume	379
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.06.026
State	Published - Sep 30 2008

Keywords

Cytoplasmic inheritance
Gene pyramiding
Genome-linked protein
Pathogenesis
Pepper pvr2 gene
Picornavirus
Protoplast printing
Tolerance
VPg
eIF4E

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2008.06.026

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title = "Complementary functions of two recessive R-genes determine resistance durability of tobacco 'Virgin A Mutant' (VAM) to Potato virus Y",

abstract = "Tobaccos VAM and NC745 carry the recessive va gene that confers resistance to PVYNN. However, they exhibit different levels of resistance durability. Upon virus inoculation, only NC745 developed sporadic systemic symptoms caused by emerging resistance-breaking variants that easily infected both NC745 and VAM genotypes. To identify the differential host conditions associated with this phenomenon, cellular accumulation, cell-to-cell movement, vascular translocation, and foliar content of PVYNN were comparatively evaluated. Virus cell-to-cell movement was restricted and its transit through the vasculature boundaries was completely blocked in both tobacco varieties. However, an additional defense mechanism operating only in tobacco VAM drastically reduced the in situ cellular virus accumulation. Genetic analyses of hybrid plant progenies indicate that VAM-type resistance was conditioned by at least two recessive genes: va and a newly reported va2 locus. Moreover, segregant plant progenies that restricted virus movement but permitted normal virus accumulation were prone to develop resistance-breaking infections.",

keywords = "Cytoplasmic inheritance, Gene pyramiding, Genome-linked protein, Pathogenesis, Pepper pvr2 gene, Picornavirus, Protoplast printing, Tolerance, VPg, eIF4E",

author = "Rodolfo Acosta-Leal and Zhongguo Xiong",

note = "Funding Information: We are grateful to Drs. Merritt Nelson (Deceased), Hans VanEtten, Judith K. Brown, and Robert McDaniel for the useful discussions during the course of this work; to Ivone Torres-Jerez for her technical advising; to Charles M. Rush for his critical reading and corrections of the manuscript; and to Becky K. Bryan and Jessica T. Smith for proofreading and editing the manuscript. RAL also thanks the Mexican Scholarship Agency, CONACyT, for financial support provided during most of his Ph.D. program.",

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AU - Acosta-Leal, Rodolfo

AU - Xiong, Zhongguo

N1 - Funding Information: We are grateful to Drs. Merritt Nelson (Deceased), Hans VanEtten, Judith K. Brown, and Robert McDaniel for the useful discussions during the course of this work; to Ivone Torres-Jerez for her technical advising; to Charles M. Rush for his critical reading and corrections of the manuscript; and to Becky K. Bryan and Jessica T. Smith for proofreading and editing the manuscript. RAL also thanks the Mexican Scholarship Agency, CONACyT, for financial support provided during most of his Ph.D. program.

PY - 2008/9/30

Y1 - 2008/9/30

N2 - Tobaccos VAM and NC745 carry the recessive va gene that confers resistance to PVYNN. However, they exhibit different levels of resistance durability. Upon virus inoculation, only NC745 developed sporadic systemic symptoms caused by emerging resistance-breaking variants that easily infected both NC745 and VAM genotypes. To identify the differential host conditions associated with this phenomenon, cellular accumulation, cell-to-cell movement, vascular translocation, and foliar content of PVYNN were comparatively evaluated. Virus cell-to-cell movement was restricted and its transit through the vasculature boundaries was completely blocked in both tobacco varieties. However, an additional defense mechanism operating only in tobacco VAM drastically reduced the in situ cellular virus accumulation. Genetic analyses of hybrid plant progenies indicate that VAM-type resistance was conditioned by at least two recessive genes: va and a newly reported va2 locus. Moreover, segregant plant progenies that restricted virus movement but permitted normal virus accumulation were prone to develop resistance-breaking infections.

AB - Tobaccos VAM and NC745 carry the recessive va gene that confers resistance to PVYNN. However, they exhibit different levels of resistance durability. Upon virus inoculation, only NC745 developed sporadic systemic symptoms caused by emerging resistance-breaking variants that easily infected both NC745 and VAM genotypes. To identify the differential host conditions associated with this phenomenon, cellular accumulation, cell-to-cell movement, vascular translocation, and foliar content of PVYNN were comparatively evaluated. Virus cell-to-cell movement was restricted and its transit through the vasculature boundaries was completely blocked in both tobacco varieties. However, an additional defense mechanism operating only in tobacco VAM drastically reduced the in situ cellular virus accumulation. Genetic analyses of hybrid plant progenies indicate that VAM-type resistance was conditioned by at least two recessive genes: va and a newly reported va2 locus. Moreover, segregant plant progenies that restricted virus movement but permitted normal virus accumulation were prone to develop resistance-breaking infections.

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KW - Tolerance

KW - VPg

KW - eIF4E

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Complementary functions of two recessive R-genes determine resistance durability of tobacco 'Virgin A Mutant' (VAM) to Potato virus Y

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Keywords

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