Comparison of transplantation of adipose tissue- and bone marrow-derived mesenchymal stem cells in the infarcted heart

Koen E.A. Van Der Bogt, Sonja Schrepfer, Jin Yu, Ahmad Y. Sheikh, Grant Hoyt, Johannes A. Govaert, Jeffrey B. Velotta, Christopher H. Contag, Robert C. Robbins, Joseph C. Wu

Research output: Contribution to journalArticlepeer-review

115 Scopus citations


Background. Mesenchymal stem cells hold promise for cardiovascular regenerative therapy. Derivation of these cells from the adipose tissue might be easier compared with bone marrow. However, the in vivo fate and function of adipose stromal cells (ASC) in the infarcted heart has never been compared directly to bone marrow-derived mesenchymal cells (MSC). Methods. ASC and MSC were isolated from transgenic FVB mice with a β-actin promoter driving firefly luciferase and green fluorescent protein double fusion reporter gene, and they were characterized using flow cytometry, microscopy, bioluminescence imaging and luminometry. FVB mice (n=8 per group) underwent myocardial infarction followed by intramyocardial injection of 5×10 5 ASC, MSC, fibroblasts (Fibro, positive control), or saline (negative control). Cell survival was measured using bioluminescence imaging for 6 weeks and cardiac function was monitored by echocardiography and pressure-volume analysis. Ventricular morphology was assessed using histology. Results. ASC and MSC were CD34 -, CD45 -, c-Kit -, CD90 +, Sca-1 +, shared similar morphology and had a population doubling time of ∼2 days. Cells expressed Fluc reporter genes in a number-dependent fashion as confirmed by luminometry. After cardiac transplantation, both cell types showed drastic donor cell death within 4 to 5 weeks. Furthermore, transplantation of either cell type was not capable of preserving ventricular function and dimensions, as confirmed by pressure-volume-loops and histology. Conclusion. This is the first study comparing the in vivo behavior of both cell types in the infarcted heart. ASC and MSC do not tolerate well in the cardiac environment, resulting in acute donor cell death and a subsequent loss of cardiac function similar to control groups.

Original languageEnglish (US)
Pages (from-to)642-652
Number of pages11
Issue number5
StatePublished - Mar 15 2009
Externally publishedYes


  • Adipose stromal cells
  • Bone marrow stem cells
  • Mesenchymal stem cells
  • Molecular imaging
  • Myocardial infarction

ASJC Scopus subject areas

  • Transplantation


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