Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Johannes Riegler; Antje Ebert; Xulei Qin; Qi Shen; Mouer Wang; Mohamed Ameen; Kazuki Kodo; Sang Ging Ong; Won Hee Lee; Grace Lee; Evgenios Neofytou; Joseph D. Gold; Andrew J. Connolly; Joseph C. Wu

doi:10.1016/j.stemcr.2015.12.008

Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Johannes Riegler
, Antje Ebert
, Xulei Qin
, Qi Shen
, Mouer Wang
, Mohamed Ameen
, Kazuki Kodo
, Sang Ging Ong
, Won Hee Lee
, Grace Lee
, Evgenios Neofytou
, Joseph D. Gold
, Andrew J. Connolly
, Joseph C. Wu

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm³. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm³ and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.

Original language	English (US)
Pages (from-to)	176-187
Number of pages	12
Journal	Stem Cell Reports
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2015.12.008
State	Published - Feb 9 2016
Externally published	Yes

Keywords

magnetic resonance imaging
microRNA biomarker
pluripotent stem cells
serum biomarker
tumorigenicity
ultrasound imaging

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2015.12.008

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Riegler, J., Ebert, A., Qin, X., Shen, Q., Wang, M., Ameen, M., Kodo, K., Ong, S. G., Lee, W. H., Lee, G., Neofytou, E., Gold, J. D., Connolly, A. J., & Wu, J. C. (2016). Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas. Stem Cell Reports, 6(2), 176-187. https://doi.org/10.1016/j.stemcr.2015.12.008

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abstract = "The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm3. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm3 and with a sensitivity of more than 87\%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.",

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author = "Johannes Riegler and Antje Ebert and Xulei Qin and Qi Shen and Mouer Wang and Mohamed Ameen and Kazuki Kodo and Ong, \{Sang Ging\} and Lee, \{Won Hee\} and Grace Lee and Evgenios Neofytou and Gold, \{Joseph D.\} and Connolly, \{Andrew J.\} and Wu, \{Joseph C.\}",

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AU - Neofytou, Evgenios

AU - Gold, Joseph D.

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Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

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