Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Johannes Riegler; Antje Ebert; Xulei Qin; Qi Shen; Mouer Wang; Mohamed Ameen; Kazuki Kodo; Sang Ging Ong; Won Hee Lee; Grace Lee; Evgenios Neofytou; Joseph D. Gold; Andrew J. Connolly; Joseph C. Wu

doi:10.1016/j.stemcr.2015.12.008

Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Johannes Riegler, Antje Ebert, Xulei Qin, Qi Shen, Mouer Wang, Mohamed Ameen, Kazuki Kodo, Sang Ging Ong, Won Hee Lee, Grace Lee, Evgenios Neofytou, Joseph D. Gold, Andrew J. Connolly, Joseph C. Wu

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm³. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm³ and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.

Original language	English (US)
Pages (from-to)	176-187
Number of pages	12
Journal	Stem Cell Reports
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2015.12.008
State	Published - Feb 9 2016
Externally published	Yes

Keywords

magnetic resonance imaging
microRNA biomarker
pluripotent stem cells
serum biomarker
tumorigenicity
ultrasound imaging

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2015.12.008

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Riegler, J., Ebert, A., Qin, X., Shen, Q., Wang, M., Ameen, M., Kodo, K., Ong, S. G., Lee, W. H., Lee, G., Neofytou, E., Gold, J. D., Connolly, A. J., & Wu, J. C. (2016). Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas. Stem Cell Reports, 6(2), 176-187. https://doi.org/10.1016/j.stemcr.2015.12.008

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abstract = "The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm3. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm3 and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.",

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AU - Neofytou, Evgenios

AU - Gold, Joseph D.

AU - Connolly, Andrew J.

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Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Abstract

Keywords

ASJC Scopus subject areas

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