TY - JOUR
T1 - Comparison of human mesenchymal stem cells derived from adipose and cord tissue
AU - Choudhery, Mahmood Saba
AU - Badowski, Michael
AU - Muise, Angela
AU - Harris, David T.
N1 - Funding Information:
This work was supported in part by the Higher Education Commission , Islamabad, Pakistan, under IRSIP and by Adicyte, Inc. The authors acknowledge the assistance of Dr J. Pierce for the adipose tissue collections and E. Hadly, RN, for the cord tissue collections.
PY - 2013/3
Y1 - 2013/3
N2 - Background aims. Stem cell therapies can provide an alternative approach for repair and regeneration of tissues and organs. Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Although bone marrow-derived MSCs have multi-lineage differentiation potential, bone marrow is not an optimal source because of the isolation process and low yield. The goal of this study was to investigate comparatively for the first time the in vitro regenerative potential of human MSCs from two other sources: umbilical cord tissue and adipose tissue. Methods. Cells from each tissue were isolated with 100% efficiency and characterized by fluorescence activated cell sorting (FACS) analysis for CD3, CD14, CD19, CD34, CD44, CD45, CD73, CD90 and CD105. Growth characteristics were investigated by population doublings, saturation density and plating efficiency. MSCs derived from both types of tissues were assessed for differentiation potential qualitatively and quantitatively. Results. FACS analysis showed no differences in expression of CD3, CD14, CD19, CD34, CD44, CD45, CD73, CD90 and CD105 between cord tissue MSCs (CT-MSCs) and adipose tissue MSCs (AT-MSCs). CT-MSCs showed more proliferative potential than AT-MSCs. When cultured in low numbers to determine colonyforming units (CFUs), CT-MSCs showed less CFUs than AT-MSCs. Cells from both sources efficiently differentiated into adipose, bone, cartilage and neuronal structures as determined with histochemistry, immunofluorescence and real-time reverse transcriptase polymerase chain reaction. Conclusions. MSCs can easily be obtained from umbilical cord and adipose tissues, and it appears that both tissues are suitable sources of stem cells for potential use in regenerative medicine.
AB - Background aims. Stem cell therapies can provide an alternative approach for repair and regeneration of tissues and organs. Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Although bone marrow-derived MSCs have multi-lineage differentiation potential, bone marrow is not an optimal source because of the isolation process and low yield. The goal of this study was to investigate comparatively for the first time the in vitro regenerative potential of human MSCs from two other sources: umbilical cord tissue and adipose tissue. Methods. Cells from each tissue were isolated with 100% efficiency and characterized by fluorescence activated cell sorting (FACS) analysis for CD3, CD14, CD19, CD34, CD44, CD45, CD73, CD90 and CD105. Growth characteristics were investigated by population doublings, saturation density and plating efficiency. MSCs derived from both types of tissues were assessed for differentiation potential qualitatively and quantitatively. Results. FACS analysis showed no differences in expression of CD3, CD14, CD19, CD34, CD44, CD45, CD73, CD90 and CD105 between cord tissue MSCs (CT-MSCs) and adipose tissue MSCs (AT-MSCs). CT-MSCs showed more proliferative potential than AT-MSCs. When cultured in low numbers to determine colonyforming units (CFUs), CT-MSCs showed less CFUs than AT-MSCs. Cells from both sources efficiently differentiated into adipose, bone, cartilage and neuronal structures as determined with histochemistry, immunofluorescence and real-time reverse transcriptase polymerase chain reaction. Conclusions. MSCs can easily be obtained from umbilical cord and adipose tissues, and it appears that both tissues are suitable sources of stem cells for potential use in regenerative medicine.
KW - Adipose tissue
KW - Cord tissue
KW - MSCs
UR - http://www.scopus.com/inward/record.url?scp=84875432362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875432362&partnerID=8YFLogxK
U2 - 10.1016/j.jcyt.2012.11.010
DO - 10.1016/j.jcyt.2012.11.010
M3 - Article
C2 - 23318344
AN - SCOPUS:84875432362
SN - 1465-3249
VL - 15
SP - 330
EP - 343
JO - Cytotherapy
JF - Cytotherapy
IS - 3
ER -