Comparison of culture-dependent and culture-independent molecular methods for characterization of vaginal microflora

Shirali Pandya, Kavitha Ravi, Vijaya Srinivas, Smitha Jadhav, Anisa Khan, Anjali Arun, Lee W. Riley, Purnima Madhivanan

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Purpose. To date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in India. Here, we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. Methodology. Culture methods involved traditional cultivation on Rogosa and sheep blood agar, whereas cultureindependent methods bypassed a culturing step by performing broadrange 16S rDNA PCR on DNA isolated directly from vaginal swabs. Results. A total of 13 women were included in the study, of which five were characterized as healthy, two were bacterial vaginosis intermediate and six were bacterial vaginosis positive according to Nugent scoring. Lactobacillus jensenii was detected most frequently when using culture methods. On the other hand, Lactobacillus iners, which was not detected by culture methods, was the most common Lactobacillus sp. detected using cultivation-independent methods. Conclusion. We found little overlap between the species found using cultivation-dependent and cultivation-independent methods. Rather, culture-dependent and culture-independent methods were found to be complementary in describing the vaginal microflora among South Indian women. Culture-independent methods were found to be superior in detecting clinically relevant vaginal flora.

Original languageEnglish (US)
Article number000407
Pages (from-to)149-153
Number of pages5
JournalJournal of Medical Microbiology
Volume66
Issue number2
DOIs
StatePublished - Feb 2017
Externally publishedYes

Keywords

  • Bacterial vaginosis
  • India
  • Lactobacillus spp.
  • Microbiome
  • Vaginal microflora

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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