TY - JOUR
T1 - Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses
AU - Pettit, C.
AU - Minnich, L. L.
AU - Shehab, Z. M.
AU - Ray, C. G.
PY - 1987
Y1 - 1987
N2 - Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.
AB - Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.
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U2 - 10.1128/jcm.25.7.1325-1326.1987
DO - 10.1128/jcm.25.7.1325-1326.1987
M3 - Article
C2 - 3038952
AN - SCOPUS:0023277632
SN - 0095-1137
VL - 25
SP - 1325
EP - 1326
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 7
ER -