Comparative survival of viruses during thermophilic and mesophilic anaerobic digestion

Hannah P. Sassi, Luisa A. Ikner, Sherif Abd-Elmaksoud, Charles P. Gerba, Ian L. Pepper

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Micro-scale technology was used to obtain survival data for three animal viruses and two bacteriophages during anaerobic digestion. The data for adenoviruses, MS2 and Φ6 provide the first published reports for survival of these viruses during mesophilic anaerobic digestion. Data were also obtained for thermophilic digestion, which showed greater inactivation of viruses at the higher temperature. Data on the survival of Φ6 are of particular interest since it is a lipid-based virus which has been suggested as a surrogate for the Ebola virus. In contrast, MS2 was found to be an inappropriate surrogate for Ebola.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalScience of the Total Environment
StatePublished - Feb 15 2018


  • Ebola
  • Lipid virus
  • Surrogate
  • Virus survival during anaerobic digestion
  • Wastewater treatment

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution


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