Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro

I. Peri; A. Shamay; M. F. McGrath; R. J. Collier; A. Gertler

doi:10.1016/S0309-1651(06)80141-4

Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro

I. Peri, A. Shamay, M. F. McGrath, R. J. Collier, A. Gertler

Research output: Contribution to journal › Article › peer-review

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Abstract

Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin.

Original language	English (US)
Pages (from-to)	359-368
Number of pages	10
Journal	Cell Biology International Reports
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/S0309-1651(06)80141-4
State	Published - Apr 1992
Externally published	Yes

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Cell Biology

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@article{e4bf391ca4b04cbe9dc3ba3a51ffdce6,

title = "Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro",

abstract = "Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin.",

author = "I. Peri and A. Shamay and McGrath, {M. F.} and Collier, {R. J.} and A. Gertler",

note = "Funding Information: We thank Dr. M. Niwa of the Fujisawa Pharmaceutical Co. (Osaka, Japan) for supplying us with IGF-I, Drs. G. Brenner and D. Hochman of the Marbek Co., for their help at the slaughter house and Mr. S. Salame for the generous gift of mammary glands. We also thank the National Hormone Pituitary Program (University of Maryland School of Medicine) for their generous gift of ovine prolactin. This investigation was supported by a grant from the National Council for Research and Development, Ministry of Science and Technology (Israel), Grant No. 2662-1-87.",

year = "1992",

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doi = "10.1016/S0309-1651(06)80141-4",

language = "English (US)",

volume = "16",

pages = "359--368",

journal = "Cell Biology International Reports",

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T1 - Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro

AU - Peri, I.

AU - Shamay, A.

AU - McGrath, M. F.

AU - Collier, R. J.

AU - Gertler, A.

N1 - Funding Information: We thank Dr. M. Niwa of the Fujisawa Pharmaceutical Co. (Osaka, Japan) for supplying us with IGF-I, Drs. G. Brenner and D. Hochman of the Marbek Co., for their help at the slaughter house and Mr. S. Salame for the generous gift of mammary glands. We also thank the National Hormone Pituitary Program (University of Maryland School of Medicine) for their generous gift of ovine prolactin. This investigation was supported by a grant from the National Council for Research and Development, Ministry of Science and Technology (Israel), Grant No. 2662-1-87.

PY - 1992/4

Y1 - 1992/4

N2 - Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin.

AB - Insulin-like growth factors (IGFs) I and II (IGF-I, IGF-II) and Des-3-IGF-I at physiological concentrations are potent mitogens of bovine undifferentiated mammary epithelial cells cultured in collagen in a serum-free medium. Des-3-IGF-I was found to be as potent as IGF-I, while IGF-II was significantly less active. All three factors acted either synergistically or additively with epidermal growth factor (EGF), cholera toxin and fetal calf serum (FCS). Indirect evidence indicates that despite its lower mitogenic activity the action of IGF-II is mediated through IGF-I receptors. The galactopoietic activity of Des-3-IGF-I and IGF-II was studied in an organ culture of bovine lactating mammary glands using lactogen-responsive fat synthesis as a test. Neither Des-3-IGF-I nor IGF-II exhibited galactopoietic activity nor did they affect the galactopoietic activity of prolactin.

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Comparative mitogenic and galactopoietic effects of IGF-I, IGF-II and DES-3-IGF-I in bovine mammary gland in vitro

Abstract

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