TY - JOUR
T1 - Comparative kidney tubule sources, isolation, perfusion, and function
AU - Beyenbach, Klaus W.
AU - Dantzler, William H.
N1 - Funding Information:
The work of the authors has been supported by grants from the National Science Foundation and the National Institutes of Health. Portions of this chapter were written by W. H. Dantzler during tenure as an Alexander yon Humboldt United States Senior Scientist Award at the University of Wfirzburg, West Germany.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - This chapter focuses on the treatment of comparative kidney tubule sources, isolation, perfusion, and function must emphasize the unique physiological systems of animals and review the experimental techniques for studying them. Accordingly, this chapter summarizes the unique renal tubular functions insofar as they are known in an invertebrate and in non-mammalian vertebrates: in an insect, and in fish, amphibians, reptiles, and birds, in that order. The Ramsay method is the most widely practiced experimental technique for studying the functions of Malpighian tubules in vitro. The isolated Malpighian tubule is placed into a known volume of Ringer under paraffin oil. The only segments of avian renal tubules perfused in vitro are the early distal tubule of the reptilian-type nephrons and the thick ascending limb of the mammalian-type nephrons from Japanese quail. Basic methods for isolation and perfusion of tubules are also presented in this chapter.
AB - This chapter focuses on the treatment of comparative kidney tubule sources, isolation, perfusion, and function must emphasize the unique physiological systems of animals and review the experimental techniques for studying them. Accordingly, this chapter summarizes the unique renal tubular functions insofar as they are known in an invertebrate and in non-mammalian vertebrates: in an insect, and in fish, amphibians, reptiles, and birds, in that order. The Ramsay method is the most widely practiced experimental technique for studying the functions of Malpighian tubules in vitro. The isolated Malpighian tubule is placed into a known volume of Ringer under paraffin oil. The only segments of avian renal tubules perfused in vitro are the early distal tubule of the reptilian-type nephrons and the thick ascending limb of the mammalian-type nephrons from Japanese quail. Basic methods for isolation and perfusion of tubules are also presented in this chapter.
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U2 - 10.1016/0076-6879(90)91014-W
DO - 10.1016/0076-6879(90)91014-W
M3 - Article
C2 - 2074756
AN - SCOPUS:0025615689
SN - 0076-6879
VL - 191
SP - 167
EP - 226
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -