Comparative analysis of mechanical stretch-induced activation activity of back and leg muscle satellite cells in vitro

Ryuichi Tatsumi, Kei Mitsuhashi, Ken Ashida, Atsushi Haruno, Akihito Hattori, Yoshihide Ikeuchi, Ronald E. Allen

    Research output: Contribution to journalArticlepeer-review

    10 Scopus citations

    Abstract

    It has previously been shown that mechanical stretch induces activation of cultured quiescent satellite cells by rapid release of hepatocyte growth factor (HGF) from its extracellular association with satellite cells and its subsequent presentation to the c-met receptor. The present study provides evidence that the stretch activation activity varies according to the origin of satellite cells from back and leg skeletal muscles in vitro. Satellite cells were isolated from three muscle groups, back (BK), upper hind limb (UL) and lower hind limb (LL) muscles, of adult male rats and stretch activation activities were compared. In response to stretch, lower hind limb satellite cells showed significantly greater response than upper hind limb and back muscles (LL > UL > BK). Immunoblots of stretched culture media revealed a higher HGF-releasing capacity of lower hind limb satellite cells than back muscle satellite cells. In addition, lower hind limb satellite cells exhibited a greater activation activity in response to exogenous HGF added to culture media than compared to satellite cells from back and upper hind limb (LL > UL > BK). The increased ability to release HGF and the increased cellular responsiveness might account for higher stretch activation activities of lower hind limb satellite cells. Electrophoretic analysis of myosin heavy chain isoforms verified a higher content of slow muscle fibers in lower limb muscles (LL > UL > BK), suggesting a difference in stretch-induced activation activity between satellite cells associated with fast and slow muscle fibers.

    Original languageEnglish (US)
    Pages (from-to)345-351
    Number of pages7
    JournalAnimal Science Journal
    Volume75
    Issue number4
    DOIs
    StatePublished - Aug 2004

    Keywords

    • Activation
    • Hepatocyte growth factor
    • Satellite cell
    • Skeletal muscle
    • Stretch

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)

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