Kombinirana primjena dekstrana i fikola smanjuje udio trombocita u populaciji mononuklearnih stanica izdvojenih iz periferne krvi pilića - Kratko priopćenje

Translated title of the contribution: Combined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear Cells - Short communication

Mladen Jergović, Gordana Nedeljković, Željko Cvetić, Željko Gottstein, Krešo Bendelja

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The ficoll density gradient method, the most widely used method for isolating avian peripheral blood mononuclear cells (PBMCs), results in significant contamination with nucleated thrombocytes. This thrombocyte contamination can affect both immunophenotyping and functional assays of T cells. We used full blood samples from ten vaccinated male layer hybrid chickens for isolating PBMCs, and have developed a low cost, combined dextran and ficoll density gradient separation method which, in comparison to the standard ficoll density gradient separation, markedly reduces thrombocyte contamination of the isolated PBMCs. An ex vivo T cell proliferation assay showed an overall reduced background proliferation in unstimulated and stimulated samples after combined dextran and ficoll separation of PBMCs, resulting in a statistically significant difference upon in vitro NDV challenge.

Translated title of the contributionCombined dextran and ficoll separation yields pure populations of chicken peripheral blood mononuclear Cells - Short communication
Original languageUndefined/Unknown
Pages (from-to)623-631
Number of pages9
JournalVeterinarski Arhiv
Volume87
Issue number5
DOIs
StatePublished - 2017
Externally publishedYes

Keywords

  • Chicken
  • Dextran
  • Ficoll
  • Peripheral blood mononuclear cells
  • Thrombocytes

ASJC Scopus subject areas

  • veterinary(all)

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