Abstract
Objective: To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment. Methods: Collagen type I extracted from rat tail tendon was combined with kerateine extracted from human hair fibers. Thermal, mechanical, and biocompatibility properties and cell behavior was assessed and compared to 100% collagen type I hydrogels to demonstrate their utility as a tissue model for 3D in vitro testing. Results: A combination (i.e., containing both collagen ‘C/KNT’) hydrogel was more thermally stable than pure collagen hydrogels and resisted thermal degradation when incubated at a hyperthermic temperature of 47°C for heating durations up to 60 min with a higher melting temperature measured by DSC. An increase in the storage modulus was only observed with an increased collagen concentration rather than an increased KTN concentration; however, a change in ECM structure was observed with greater fiber alignment and width with an increase in KTN concentration. The C/KTN hydrogels, specifically 50/50 C/KTN hydrogels, also supported the growth and of fibroblasts and MDA-MB-231 breast cancer cells similar to those seeded in 100% collagen hydrogels. Conclusion: This multi-protein C/KTN hydrogel shows promise for future studies involving thermal stress studies without compromising the 3D ECM environment or cell growth.
Original language | English (US) |
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Pages (from-to) | 830-845 |
Number of pages | 16 |
Journal | International Journal of Hyperthermia |
Volume | 38 |
Issue number | 1 |
DOIs | |
State | Published - 2021 |
Keywords
- Hydrogel
- biomimetic engineering
- collagen
- hyperthermia
- kerateine
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
- Cancer Research
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Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
Zuniga, K. (Creator), Gadde, M. (Creator), Scheftel, J. (Creator), Senecal, K. (Creator), Cressman, E. (Creator), Van Dyke, M. E. (Creator) & Rylander, M. N. (Creator), Taylor & Francis, 2021
DOI: 10.6084/m9.figshare.14709241.v1, https://tandf.figshare.com/articles/dataset/Collagen_kerateine_multi-protein_hydrogels_as_a_thermally_stable_extracellular_matrix_for_3D_i_in_vitro_i_models/14709241/1
Dataset
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Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models
Zuniga, K. (Creator), Gadde, M. (Creator), Scheftel, J. (Creator), Senecal, K. (Creator), Cressman, E. (Creator), Van Dyke, M. E. (Creator) & Rylander, M. N. (Creator), Taylor & Francis, 2021
DOI: 10.6084/m9.figshare.14709241, https://tandf.figshare.com/articles/dataset/Collagen_kerateine_multi-protein_hydrogels_as_a_thermally_stable_extracellular_matrix_for_3D_i_in_vitro_i_models/14709241
Dataset