Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

Mousumi Ghosh; Xiaoyan Song; Ghassan Mouneimne; Mazen Sidani; David S. Lawrence; John S. Condeelis

doi:10.1126/science.1094561

Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

Mousumi Ghosh, Xiaoyan Song, Ghassan Mouneimne, Mazen Sidani, David S. Lawrence, John S. Condeelis

Research output: Contribution to journal › Article › peer-review

572 Scopus citations

Abstract

A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.

Original language	English (US)
Pages (from-to)	743-746
Number of pages	4
Journal	Science
Volume	304
Issue number	5671
DOIs	https://doi.org/10.1126/science.1094561
State	Published - Apr 30 2004
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1094561

Cite this

@article{bf906fe9358f4c3586072df877d55a5a,

title = "Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility",

abstract = "A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.",

author = "Mousumi Ghosh and Xiaoyan Song and Ghassan Mouneimne and Mazen Sidani and Lawrence, {David S.} and Condeelis, {John S.}",

year = "2004",

month = apr,

day = "30",

doi = "10.1126/science.1094561",

language = "English (US)",

volume = "304",

pages = "743--746",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5671",

}

TY - JOUR

T1 - Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

AU - Ghosh, Mousumi

AU - Song, Xiaoyan

AU - Mouneimne, Ghassan

AU - Sidani, Mazen

AU - Lawrence, David S.

AU - Condeelis, John S.

PY - 2004/4/30

Y1 - 2004/4/30

N2 - A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.

AB - A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.

UR - http://www.scopus.com/inward/record.url?scp=2342436150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342436150&partnerID=8YFLogxK

U2 - 10.1126/science.1094561

DO - 10.1126/science.1094561

M3 - Article

C2 - 15118165

AN - SCOPUS:2342436150

SN - 0036-8075

VL - 304

SP - 743

EP - 746

JO - Science

JF - Science

IS - 5671

ER -

Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this