Co-culture of gastric organoids and immortalized stomach mesenchymal cells

Nina Bertaux-Skeirik; Jomaris Centeno; Rui Feng; Michael A. Schumacher; Ramesh A. Shivdasani; Yana Zavros

doi:10.1007/978-1-4939-3603-8_3

Co-culture of gastric organoids and immortalized stomach mesenchymal cells

Nina Bertaux-Skeirik
, Jomaris Centeno
, Rui Feng
, Michael A. Schumacher
, Ramesh A. Shivdasani
, Yana Zavros

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

Original language	English (US)
Pages (from-to)	23-31
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1422
DOIs	https://doi.org/10.1007/978-1-4939-3603-8_3
State	Published - 2016
Externally published	Yes

Keywords

Co-culture
Immortalized stomach mesenchymal cells
Mouse fundic gastric organoids

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-3603-8_3

Cite this

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title = "Co-culture of gastric organoids and immortalized stomach mesenchymal cells",

abstract = "Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.",

keywords = "Co-culture, Immortalized stomach mesenchymal cells, Mouse fundic gastric organoids",

author = "Nina Bertaux-Skeirik and Jomaris Centeno and Rui Feng and Schumacher, \{Michael A.\} and Shivdasani, \{Ramesh A.\} and Yana Zavros",

note = "Funding Information: This work was supported by NIHNational Institutes of Health1R01DK083402 grant (Zavros), NIH 5T32GM105526 grant (Bertaux-Skeirik), and the Albert J. Ryan Fellowship (Bertaux-Skeirik). Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

doi = "10.1007/978-1-4939-3603-8\_3",

language = "English (US)",

volume = "1422",

pages = "23--31",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

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T1 - Co-culture of gastric organoids and immortalized stomach mesenchymal cells

AU - Bertaux-Skeirik, Nina

AU - Centeno, Jomaris

AU - Feng, Rui

AU - Schumacher, Michael A.

AU - Shivdasani, Ramesh A.

AU - Zavros, Yana

N1 - Funding Information: This work was supported by NIHNational Institutes of Health1R01DK083402 grant (Zavros), NIH 5T32GM105526 grant (Bertaux-Skeirik), and the Albert J. Ryan Fellowship (Bertaux-Skeirik). Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

AB - Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

KW - Co-culture

KW - Immortalized stomach mesenchymal cells

KW - Mouse fundic gastric organoids

UR - https://www.scopus.com/pages/publications/84990057354

UR - https://www.scopus.com/pages/publications/84990057354#tab=citedBy

U2 - 10.1007/978-1-4939-3603-8_3

DO - 10.1007/978-1-4939-3603-8_3

M3 - Article

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AN - SCOPUS:84990057354

SN - 1064-3745

VL - 1422

SP - 23

EP - 31

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Co-culture of gastric organoids and immortalized stomach mesenchymal cells

Abstract

Keywords

ASJC Scopus subject areas

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