TY - JOUR
T1 - Co-culture of gastric organoids and immortalized stomach mesenchymal cells
AU - Bertaux-Skeirik, Nina
AU - Centeno, Jomaris
AU - Feng, Rui
AU - Schumacher, Michael A.
AU - Shivdasani, Ramesh A.
AU - Zavros, Yana
N1 - Funding Information:
This work was supported by NIHNational Institutes of Health1R01DK083402 grant (Zavros), NIH 5T32GM105526 grant (Bertaux-Skeirik), and the Albert J. Ryan Fellowship (Bertaux-Skeirik).
Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016
Y1 - 2016
N2 - Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.
AB - Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.
KW - Co-culture
KW - Immortalized stomach mesenchymal cells
KW - Mouse fundic gastric organoids
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U2 - 10.1007/978-1-4939-3603-8_3
DO - 10.1007/978-1-4939-3603-8_3
M3 - Article
C2 - 27246019
AN - SCOPUS:84990057354
SN - 1064-3745
VL - 1422
SP - 23
EP - 31
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -