Co-culture of gastric organoids and immortalized stomach mesenchymal cells

Nina Bertaux-Skeirik, Jomaris Centeno, Rui Feng, Michael A. Schumacher, Ramesh A. Shivdasani, Yana Zavros

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalMethods in Molecular Biology
StatePublished - 2016
Externally publishedYes


  • Co-culture
  • Immortalized stomach mesenchymal cells
  • Mouse fundic gastric organoids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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