Cloning, sequencing, and expression of the gene coding for the human platelet α2-adrenergic receptor

B. K. Kobilka; H. Matsui; T. S. Kobilka; T. L. Yang-Feng; U. Francke; M. G. Caron; R. J. Lefkowitz; J. W. Regan

doi:10.1126/science.2823383

Cloning, sequencing, and expression of the gene coding for the human platelet α₂-adrenergic receptor

B. K. Kobilka, H. Matsui, T. S. Kobilka, T. L. Yang-Feng, U. Francke, M. G. Caron, R. J. Lefkowitz, J. W. Regan

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Abstract

The gene for the human platelet α₂-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of α₂-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human β₂- and β₁-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional α₂-adrenergic receptor subtypes.

Original language	English (US)
Pages (from-to)	650-656
Number of pages	7
Journal	Science
Volume	238
Issue number	4827
DOIs	https://doi.org/10.1126/science.2823383
State	Published - 1987
Externally published	Yes

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title = "Cloning, sequencing, and expression of the gene coding for the human platelet α2-adrenergic receptor",

abstract = "The gene for the human platelet α2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of α2-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human β2- and β1-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional α2-adrenergic receptor subtypes.",

author = "Kobilka, {B. K.} and H. Matsui and Kobilka, {T. S.} and Yang-Feng, {T. L.} and U. Francke and Caron, {M. G.} and Lefkowitz, {R. J.} and Regan, {J. W.}",

year = "1987",

doi = "10.1126/science.2823383",

language = "English (US)",

volume = "238",

pages = "650--656",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4827",

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T1 - Cloning, sequencing, and expression of the gene coding for the human platelet α2-adrenergic receptor

AU - Kobilka, B. K.

AU - Matsui, H.

AU - Kobilka, T. S.

AU - Yang-Feng, T. L.

AU - Francke, U.

AU - Caron, M. G.

AU - Lefkowitz, R. J.

AU - Regan, J. W.

PY - 1987

Y1 - 1987

N2 - The gene for the human platelet α2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of α2-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human β2- and β1-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional α2-adrenergic receptor subtypes.

AB - The gene for the human platelet α2-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of α2-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human β2- and β1-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional α2-adrenergic receptor subtypes.

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Cloning, sequencing, and expression of the gene coding for the human platelet α₂-adrenergic receptor

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