TY - JOUR
T1 - Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis
AU - Huang, Jianfeng
AU - Zhang, Jian
AU - Lei, Ting
AU - Chen, Xiaodong
AU - Zhang, Yan
AU - Zhou, Lulu
AU - Yu, An
AU - Chen, Zhilong
AU - Zhou, Ronghua
AU - Yang, Zaiqing
PY - 2010/7
Y1 - 2010/7
N2 - Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPARγ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPARγ, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.
AB - Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPARγ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPARγ, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.
KW - Chemerin
KW - Chemerin receptors
KW - Lipogenesis
KW - Porcine
KW - Transcriptional regulation
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U2 - 10.3858/BMBRep.2010.43.7.491
DO - 10.3858/BMBRep.2010.43.7.491
M3 - Article
C2 - 20663411
AN - SCOPUS:77956552969
SN - 1976-6696
VL - 43
SP - 491
EP - 498
JO - BMB Reports
JF - BMB Reports
IS - 7
ER -