Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein

Torsten Falk; Wolfgang meyerhof; Brian J. Corrette; Judith Schäfer; Christiane K. Bauer; Jürgen R. Schwarz; Dietmar Richter

doi:10.1016/0014-5793(95)00527-G

Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein

Torsten Falk, Wolfgang meyerhof, Brian J. Corrette, Judith Schäfer, Christiane K. Bauer, Jürgen R. Schwarz, Dietmar Richter

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

In GH₃/B₆ cells at least two different inward K⁺ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K⁺ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH₃/B₆ cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.

Original language	English (US)
Pages (from-to)	127-131
Number of pages	5
Journal	FEBS Letters
Volume	367
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)00527-G
State	Published - Jun 26 1995
Externally published	Yes

Keywords

Anterior pituitary
GH/B cells
Human embryonic kidney cell expression
Hybridization, in situ
Oocyte expression
Prolactin secretion

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)00527-G

Cite this

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title = "Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein",

abstract = "In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.",

keywords = "Anterior pituitary, GH/B cells, Human embryonic kidney cell expression, Hybridization, in situ, Oocyte expression, Prolactin secretion",

author = "Torsten Falk and Wolfgang meyerhof and Corrette, {Brian J.} and Judith Sch{\"a}fer and Bauer, {Christiane K.} and Schwarz, {J{\"u}rgen R.} and Dietmar Richter",

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T1 - Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein

AU - Falk, Torsten

AU - meyerhof, Wolfgang

AU - Corrette, Brian J.

AU - Schäfer, Judith

AU - Bauer, Christiane K.

AU - Schwarz, Jürgen R.

AU - Richter, Dietmar

PY - 1995/6/26

Y1 - 1995/6/26

N2 - In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.

AB - In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.

KW - Anterior pituitary

KW - GH/B cells

KW - Human embryonic kidney cell expression

KW - Hybridization, in situ

KW - Oocyte expression

KW - Prolactin secretion

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Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein

Abstract

Keywords

ASJC Scopus subject areas

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