Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan

Nai Tong Yu; Yu Liang Zhang; Tuan Cheng Feng; Jian Hua Wang; Mahesh Kulye; Wen Jun Yang; Zhan Song Lin; Zhongguo Xiong; Zhi Xin Liu

doi:10.1007/s11262-012-0718-9

Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan

Nai Tong Yu, Yu Liang Zhang, Tuan Cheng Feng, Jian Hua Wang, Mahesh Kulye, Wen Jun Yang, Zhan Song Lin, Zhongguo Xiong, Zhi Xin Liu

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The genome of Banana bunchy top virus (BBTV) consists of six segments of single-stranded DNA of approximately 1 kb in length. We identified and sequenced the complete genomes of two BBTV isolates, one with and one without satellite DNA, from Haikou, Hainan, China. The Haikou-2 isolate contains six genomic segments and an additional satellite DNA while the Haikou-4 isolate contains only six genomic segments. Typical of other babuviruses, each genomic segment encodes a single open reading frame and contains the highly conserved stem-loop and major common regions. Phylogenetic analysis of the two Haikou isolates together with existing sequence records in GenBank confirmed the grouping of BBTV into two large groups and further refined the geographical distribution of each group. To accommodate the changes in the BBTV geographical distribution, the two groups are proposed as the Southeast Asian group and the Pacific-Indian Oceans group. Both the Haikou-2 and Haikou-4 isolates belong to the newly proposed Southeast Asian group.

Original language	English (US)
Pages (from-to)	488-494
Number of pages	7
Journal	Virus Genes
Volume	44
Issue number	3
DOIs	https://doi.org/10.1007/s11262-012-0718-9
State	Published - Jun 2012

Keywords

BBTV
Cloning
Genome
Phylogeny
Sequencing

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

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10.1007/s11262-012-0718-9

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title = "Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan",

abstract = "The genome of Banana bunchy top virus (BBTV) consists of six segments of single-stranded DNA of approximately 1 kb in length. We identified and sequenced the complete genomes of two BBTV isolates, one with and one without satellite DNA, from Haikou, Hainan, China. The Haikou-2 isolate contains six genomic segments and an additional satellite DNA while the Haikou-4 isolate contains only six genomic segments. Typical of other babuviruses, each genomic segment encodes a single open reading frame and contains the highly conserved stem-loop and major common regions. Phylogenetic analysis of the two Haikou isolates together with existing sequence records in GenBank confirmed the grouping of BBTV into two large groups and further refined the geographical distribution of each group. To accommodate the changes in the BBTV geographical distribution, the two groups are proposed as the Southeast Asian group and the Pacific-Indian Oceans group. Both the Haikou-2 and Haikou-4 isolates belong to the newly proposed Southeast Asian group.",

keywords = "BBTV, Cloning, Genome, Phylogeny, Sequencing",

author = "Yu, {Nai Tong} and Zhang, {Yu Liang} and Feng, {Tuan Cheng} and Wang, {Jian Hua} and Mahesh Kulye and Yang, {Wen Jun} and Lin, {Zhan Song} and Zhongguo Xiong and Liu, {Zhi Xin}",

note = "Funding Information: Acknowledgments This study was funded by the Chinese National Natural Science Foundation (31070131), the {\textquoteleft}{\textquoteleft}211 project{\textquoteright}{\textquoteright} for Hainan University, and the Chinese National Nonprofit Institute Research Grant to CATAS-ITBB (ITBB110303).",

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doi = "10.1007/s11262-012-0718-9",

language = "English (US)",

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pages = "488--494",

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T1 - Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan

AU - Yu, Nai Tong

AU - Zhang, Yu Liang

AU - Feng, Tuan Cheng

AU - Wang, Jian Hua

AU - Kulye, Mahesh

AU - Yang, Wen Jun

AU - Lin, Zhan Song

AU - Xiong, Zhongguo

AU - Liu, Zhi Xin

N1 - Funding Information: Acknowledgments This study was funded by the Chinese National Natural Science Foundation (31070131), the ‘‘211 project’’ for Hainan University, and the Chinese National Nonprofit Institute Research Grant to CATAS-ITBB (ITBB110303).

PY - 2012/6

Y1 - 2012/6

N2 - The genome of Banana bunchy top virus (BBTV) consists of six segments of single-stranded DNA of approximately 1 kb in length. We identified and sequenced the complete genomes of two BBTV isolates, one with and one without satellite DNA, from Haikou, Hainan, China. The Haikou-2 isolate contains six genomic segments and an additional satellite DNA while the Haikou-4 isolate contains only six genomic segments. Typical of other babuviruses, each genomic segment encodes a single open reading frame and contains the highly conserved stem-loop and major common regions. Phylogenetic analysis of the two Haikou isolates together with existing sequence records in GenBank confirmed the grouping of BBTV into two large groups and further refined the geographical distribution of each group. To accommodate the changes in the BBTV geographical distribution, the two groups are proposed as the Southeast Asian group and the Pacific-Indian Oceans group. Both the Haikou-2 and Haikou-4 isolates belong to the newly proposed Southeast Asian group.

AB - The genome of Banana bunchy top virus (BBTV) consists of six segments of single-stranded DNA of approximately 1 kb in length. We identified and sequenced the complete genomes of two BBTV isolates, one with and one without satellite DNA, from Haikou, Hainan, China. The Haikou-2 isolate contains six genomic segments and an additional satellite DNA while the Haikou-4 isolate contains only six genomic segments. Typical of other babuviruses, each genomic segment encodes a single open reading frame and contains the highly conserved stem-loop and major common regions. Phylogenetic analysis of the two Haikou isolates together with existing sequence records in GenBank confirmed the grouping of BBTV into two large groups and further refined the geographical distribution of each group. To accommodate the changes in the BBTV geographical distribution, the two groups are proposed as the Southeast Asian group and the Pacific-Indian Oceans group. Both the Haikou-2 and Haikou-4 isolates belong to the newly proposed Southeast Asian group.

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KW - Phylogeny

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Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan

Abstract

Keywords

ASJC Scopus subject areas

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